Olympus America

Press Center

Olympus 150x 1.45 NA TIRF Objective Offers Outstanding Light Transmission from UV to IR

MELVILLE, NY, September 15, 2005 - A new 150x, 1.45 NA oil objective offers excellent light transmission for total internal reflectance fluorescence (TIRF) research microscopy over an unprecedented range of the spectrum from the ultraviolet (UV) to the infrared (IR). The high-NA Olympus U Apochromat 150X TIRF objective enables easy TIRF observation using a standard coverglass and standard immersion oil. Olympus offers the most comprehensive line of TIRF optics for advanced research.

The objective is the only one currently available that offers both high UV transmission down to 330 nm and a very high numerical aperture, making it possible to perform TIRF microscopy and UV applications such as un-caging and/or photo-activation with just one lens. In addition, the objective offers IR transmission well beyond 1000 nm, making it well suited to IR fluorescence imaging and IR laser applications, and opening up the potential for live-cell microscopy and future applications in the growing IR microscopy field.

The high magnification of the objective enhances small specimen structures on the CCD camera, thus overcoming the resolution limit imposed by the CCDís pixel size, an advantage because it does not add either the light loss or potential for aberrations that additional magnification optics can cause. In addition, due to its small field of view, the opticís illumination light is concentrated onto a very small area of the specimen, offering added illumination intensity that makes the objective a good choice for single molecule fluorescence applications.

The 150x NA 1.45 objective offers evanescent wave penetration characteristics similar to the existing Olympus 60x NA 1.45 TIRF objective. It has a working distance of 0.10, a field number of 22, and uses a correction collar to compensate for coverglass thickness and temperature.

For more information on the Olympus U Apochromat 150X TIRF objective , contact Olympus America Inc., Two Corporate Center Drive, P.O. Box 9058, Melville, NY 11747-9058; www.olympusamerica.com/microscopes ; Email: olympusseg@econnextions.com; or phone 1-800-455-8236.

About Olympus Scientific Equipment Group
Olympus Scientific Equipment Group provides innovative microscope imaging solutions for researchers, doctors, clinicians and educators. Olympus microscope systems offer unsurpassed optics, superior construction and system versatility to meet the ever-changing needs of microscopists, paving the way for future advances in life science.

About Olympus
Olympus is a precision technology leader, designing and delivering innovative solutions in healthcare and consumer electronics worldwide.

Olympus works collaboratively with its customers and its parent company, Tokyo-based Olympus Corporation, to leverage R&D investment in precision technology and manufacturing processes across diverse business lines. These include:

  • Gastrointestinal endoscopes, accessories, and minimally invasive surgical products;
  • Advanced clinical and research microscopes;
  • Lab automation systems, chemistry-immuno and blood bank analyzers and reagents; and
  • Digital and film cameras, and digital voice recorders.

In the U.S. and Canada, Olympus serves healthcare, scientific and commercial laboratory markets with integrated product solutions and financial, educational and consulting services that help customers efficiently, reliably, safely, and easily achieve superior results. Olympus is the leader in gastrointestinal endoscopy and clinical and educational microscopes. The company's market-leading consumer electronics business spans North and South America. For more information, visit www.olympusamerica.com.