Optimized for Imaging Live Cells and Tissues, FV1200 brings sensitivity and power to your research through a range of innovative technology and sensitivity improvements, allowing you to capture the most critical elements of your biological samples with speed, precision and reliability.
FV1200 Specifications
| Spectral type fluorescence detector | Filter type fluorescence detector | |||
| Laser light | Ultraviolet/Visible light laser | LD laser: 405nm: 50mW, 440nm: 25mW, 473nm: 15mW, 559nm: 15mW, 635mW, 20mW Multiline Argon laser (457nm, 488nm, 515nm, Total 30mW), HeNe(G) laser (543nm, 1mW) |
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| AOTF laser combiner | Visible light laser platform with implemented AOTF system, Ultra-fast intensity modulation with individual laser lines, additional shutter control Continuously variable (0.1% - 100%, 0.1% increment), REX: Capable of laser intensity adjustment and laser wavelength selection for each region. |
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| Fiber | Broadband type (400nm-650nm) | |||
| Scanning and Detection | Scanner module | Standard 3 laser ports, VIS - UV - IR Excitation dichromatic mirror turret, 6 position (High performance DMs and 20/80 half mirror), Dual galvanometer mirror scanner (X, Y). Anti-oxidization silver coating ensures durability and improves reflection efficiency in both excitation and emission with the set of two galvanometer scanning mirrors, increasing the reflectance in the visible range by 5-15%, and IR reflectance up to 22%. |
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| Detector module | Standard 3 confocal Channels (3 photomultiplier detectors). Special selection of GaAsP PMT detectors offers high sensitivity with up to 45% quantum efficiency (QE). The combination of GaAsP PMT for high sensitivity and standard multi-alkali PMTs for high dynamic range offers the user experimental flexibility across a wide spectral range. Additional optional output port light path available for optional units 6 position beamsplitter turrets with CH1 and CH2 CH1 and CH2 equipped with independent grating and slit for fast and flexible spectral detection Selectable wavelength bandwidth: 1-100nm Wavelength resolution: 2nm. Wavelength switching speed: 100nm/msec CH3 with 6 position barrier filter turret. | Standard 3 confocal Channels (3 photomultiplier detectors) Additional optional output port light path available for optional units 6 position beamsplitter turrets with CH1 and CH2 CH1 to CH3 each with 6 position barrier filter turret (High performance filters). | ||
| Filters | High performance sputtered filters, dichromatic mirrors and barrier filters. | |||
| Scanning method | 2 galvanometer scanning mirrors | |||
| Scanning modes | Pixel size: 64 x 64 - 4096 x 4096
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| X,Y,T,Z,λ Line scanning: Straight line with free orientation, free line, Point scanning. | X,Y,T,Z Line scanning: Straight line with free orientation, free line, Point scanning | |||
| Photo detection method | 2 detection modes: Analog integration and hybrid photon counting | |||
| Pinhole | Single motorized pinhole pinhole diameter ø50 - 300μm (0.5μm step) |
Single motorized pinhole pinhole diameter ø50 - 800μm (0.5μm step) |
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| Field Number (NA) | 18 | |||
| Optical Zoom | 1x - 50x in 0.1x increment | |||
| Z-drive | Integrated motorized focus module of the microscope, minimum increment 0.01μm or 10 nm | |||
| Transmitted light detector unit | Module with integrated external transmitted light photomultiplier detector and 100W Halogen lamp, motorized switching, fiber adaptation to microscope frame. | |||
| Microscope | Motorized microscope | Inverted IX81 or IX83, Upright BX61, Upright focusing nosepiece & fixed stage BX61WI | ||
| Fluorescence illumination unit | External fluorescence light source with motorized shutter, fiber adaptation to optical port of scan unit Motorized switching between LSM light path and fluorescence illumination |
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| System Control | PC | PC-AT compatible, OS: Windows 7 Professional (English version), Memory: 4GB or larger, CPU:XEON 3.6GHz or higher, Hard disk: 1TB or larger, Media: Blue Ray, FV1000 Special I/F board (built-in PC), Graphic board: QUADRO 600 ATX | ||
| Power Supply Unit | Galvo control boards, scanning mirrors and gratings, Real time controller | Galvo control boards, scanning mirrors | ||
| Monitor | Monitor: SXGA 1280X1024, dual 19 inch or larger monitors | |||
| Optional unit | SIM Scanner | 2 galvanometer scanning mirrors, pupil projection lens, built-in laser shutter, 1 laser port Fiber introduction of near UV diode laser or visible light laser, Optional: 2nd AOTF laser combiner | ||
| TIRFM unit | Available laser: 405~633nm. Motorized penetration ratio adjustment. Automatic optical setting for TIRFM objectives. | |||
| 4th CH detector | Module with photomultiplier detector, barrier filter turret, beamsplitter turret mounted with 3rd CH light path. |
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| Fiber port for fluorescence | Output port equipped with FC fiber connector (compatible fiber core 100 -125μm) | |||
| Software Display | Dual monitor display |
| Optional Hardware | SIM scanner unit, 4ch detector. Fiber port |
| Image format | OIB/OIF image format 8/16 bit gray scale/index color, 24/32/48 bit color, JPEG/BMP/TIFF/AVI/MOV image functions Olympus multi-tif format |
| Image acquisition | Region designation: point, line, free line, clip, clip zoom Real-time image calculation: Kalman filtering, photon counting mode |
| 2-dimension: XY, XZ, XT and Xλ 3-dimension: XYZ, XYT, XYλ, XZT, XTλ and XZλ 4-dimension: XYZT, XZTλ and XYTλ | |
| Image pixel format | 64 x 64 - 4096 x 4096 |
| Programmable Time Controller function | Time Controller function |
| Image display | Each image display: Single-channel side-by-side, merge, cropping, live tiling, live tile, series (Z/T/λ), LUT: individual color setting, pseudo-color, comment: graphic and text input |
| 3D visualization and observation | Interactive volume rendering Free orientation of cross section display 3D animation, left/right stereo pairs, red/green stereoscopic images and cross section 3D and 2D sequential operation function |
| Spectral unmixing | 2 Fluorescence spectral unmixing modes (normal and blind mode) |
| Image processing | Individual filter: average, low-pass, Sobel, Median, *Prewitt, 2D Laplacian, edge enhancement etc. Calculations: inter-image, mathematical and logical, DIC background leveling |
| Image analysis | Fluorescence intensity, area and perimeter measurement, time-lapse measurement |
| Statistical processing | 2D data histogram display, colocalization |
| Others (options) | Off-line review station software |
Objectives for BX and IX (using U-UCD8A, IX-LWUCDA and U-DICTS)
| Description | NA | W.D. | Cover glass thickness | Immersion | Correction ring | Condenser for BX U-UCD8A optical element | Condenser for IX IX-LWUCDA optical element | U-DICTS position |
| UPLSAPO 4x | 0.16 | 13 | - | |||||
| UPLSAPO 10x | 0.40 | 3.1 | 0.17 | U-DIC10 | IX2-DIC10 | normal | ||
| UPLSAPO 10xO3 | 0.40 | 0.24 | 0.17 | Oil | U-DP10 | IX-DP10 | normal | |
| UPLSAPO 10xW3 | 0.40 | 0.43 | 0.17 | Water | U-DP10 | IX-DP10 | normal | |
| UPLSAPO 20x | 0.75 | 0.6 | 0.17 | U-DIC20 | IX2-DIC20 | normal | ||
| UPLSAPO 20xO | 0.85 | 0.17 | - | Oil | U-DIC20 | IX2-DIC20 | normal | |
| UPLSAPO 40x | 0.90 | 0.2 | 0.11-0.23 | O | U-DIC40 | IX2-DIC40 | normal | |
| UPLSAPO 40xO | 1.30 | 0.2 | 0.17 | Oil | U-DIC40 | IX2-DIC40 | BFP1 | |
| PLAPON 60xOTIRFM | 1.45 | 0.10 | 0.17 | Oil | U-DIC60 | IX2-DIC60 | BFP1 | |
| UPLSAPO 60xO | 1.35 | 0.15 | 0.17 | Oil | U-DIC60 | IX2-DIC60 | BFP1 | |
| UPLSAPO 60xW | 1.20 | 0.28 | 0.15-0.2 | Water | O | U-DIC60 | IX2-DIC60 | normal |
| UPLSAPO 100xO | 1.40 | 0.12 | 0.17 | Oil | U-DIC100 | IX2-DIC100 | normal |
Objectives for fixed stage upright microscopes (using WI-UCD, WI-DICTHRA)
| Objective | NA | W.D. | DIC prism | Revolving nosepiece |
| MplanN5x | 0.10 | 20.0 | - | WI-SSNP, WI-SRE2 |
| UMPlanFl10xW | 0.30 | 3.30 | U-LDPW10H | WI-SSNP, WI-SRE2 |
| UMPlanFl20xW | 0.50 | 3.30 | U-LDPW20H | WI-SSNP, WI-SRE2 |
| LUMPlanFl40xW | 0.80 | 3.30 | U-LDPW40H | WI-SSNP, WI-SRE2 |
| LUMPlanFl60xW | 0.90 | 2.00 | U-LDPW60H | WI-SSNP, WI-SRE2 |
| LUMPlanFl40xW/IR2 | 0.80 | 3.30 | U-LDPW40H | WI-SSNP, WI-SRE2 |
| LUMPlanFl60xW/IR2 | 0.90 | 2.00 | U-LDPW60H | WI-SSNP, WI-SRE2 |
| LUMPlanFl100xW | 1.00 | 1.50 | U-LDPW60H | WI-SSNP, WI-SRE2 |
| XLUMPlanFl20xW | 0.95* | 2.00 | U-LDPXLU20HR | WI-SNPXLU |
* Note: These conditions are not met in confocal microscopy
Dimensions, weight and power consumption
| Dimensions(mm) | Weight (kg) | Power consumption | ||
| Microscope withScan unit | BX system: 320(W) x 580(D) x 565(H) | 41 | - |
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| IX system: 350(W) x 750(D) x 640(H) | 59 | |||
| Fluorescence illumination unit | Lamp: 180(W) x 320(D) x 235(H) | 6.7 | 100-240V 50/60 Hz 1.6A | |
| Power supply: 90(W) x 270(D) x 180(H) | 3.0 | |||
| Transmitted light detection unit | 170(W) x 330(D) x 130(H) | 5.9 | - |
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| Microscope control unit | 125(W) x 332(D) x 216(H) | 5.2 | AC 100-120/220-240V 50/60 Hz 3.5A/1.5A | |
| FV Power Supply unit | 180(W) x 328(D) x 424(H) | 7.5 | AC 100-120/220-240V 50/60 Hz 4.0A/2.0 A | |
| FV Control unit (PC) | 136(W) x 380(D) x 329(H) | 8.5 | AC 100/240 V 50/60 Hz 600 W | |
| Display | 19 inch, due (value per monitor) | 363(W) x 216(D) x 389.5-589.5(H) | 5.9 | AC 100-120/200-240 V 50/60 Hz 0.65 A/0.4 A |
| 29.7 inch | 694 (W) x 276 (D) x 489–589 (H) | 13.0 | AC 100-240 V 50/60Hz 2.0 A | |
| Power supply unit for laser combiner | 210(W) x 300(D) X 100(H) | 4.0 | AC 100-120/200-240 V 50/60 Hz 2.0 A/1.0 A | |
| Laser combiner (with Laser heads) | 514(W) x 504(D) x 236(H) | 45 | - |
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| Laser combiner (without Ar laser heads) | 514(W) x 364(D) x 236(H) | 40 | - |
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| LD559 laser power supply | 200(W) x 330(D) x 52(H) | 1.2 | AC 100-240 V 50/60 Hz 30 W | |
| Multi Ar laser Power Supply | 162(W) x 287(D) x 91(H) | 4.4 | AC 100-240 V 50/60 Hz 20 A | |
| HeNe G laser Power Supply | 130(W) x 224(D) x 62(H) | 1.8 | AC 100-120 V 50/60 Hz 0.45 A | |
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