The FV1200MPE TWIN is the ultimate multiphoton system for optogenetics, electrophysiology and high speed measurements in live tissues. With dual IR laser inputs, an increase of 50% in multiphoton excitation efficiency and superb MPE dedicated objectives, the FV1200MPE TWIN delivers high sensitivity and deep imaging with the ability to simultaneously image and stimulate with both pulsed IR and visible lasers. Configured with standard visible confocal capability and a range of descanned and non-descanned detector options, the FV1200MPE twin pushes the envelope for interactive visible and multiphoton imaging.
SPECIFICATIONS
| B System | S System | T System | M System | ||
| Laser unit | IR pulsed laser with negative chirp for multiphoton excitation |
•Mode-locked Ti : sapphire laser [femtosecond laser (equipped with a group velocity compensation)], laser power unit, water-cooled circulating chiller |
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Visible light laser AOTF laser combiner |
LD laser: 405nm: 50mW, 440nm: 25mW, 473nm 15mW, 559nm 15mW, 635nm 20mW AOTF laser combiner Multi Ar laser (457nm, 488nm, 515nm, Total 30mW), HeNe (G) laser (543nm, 1mW) Modulation: Continuously adjustable via an AOTF (0.1 — 100% in 0.1% increments)
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N/A | |||
| Single laser for visible light | LD473 laser (15mW) Depending on the type of modulation: light intensity modulation, shutter control, connected to the scanner via single-mode optical fiber. | N/A | |||
| Scanning Unit | Scanning method | Light deflection via 2 falvanometer scanning mirrors | |||
| Scanning modes | •Pixel size: 64 x 64 — 4096 x 4096 pixels Scanning speed: (pixel time): 2μs — 200μs High-speed scanning mode: 16 frames/s (256 x 256)
•Dimensions: Time, Z, (wavelength) (or any combination thereof) |
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| Zoom size | Observation position zoom with inclination width modification of galvanometer mirror: 1-50x (adjustable in 0.1x increments. | ||||
| Confocal detector (The M scanner does not have a confocal detector) |
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| Optics with infrared laser for multiphoton imaging |
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| Component incorporating the laser for multiphoton imaging | Main scanner for observation |
ASU scanner for laser light stimulation, Main Scanner for observation: VIS laser |
Incorporating 2 independent lasers for laser light stimulation/observation |
M scanner for observation |
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| Detector for multiphoton imaging | Reflected light fluorescent detector | Photomultiplier (2 or 4 channels), Fluorescence wavelength can be selected with teh dedicated filter cube (replaceable) | |||
| Transmitted light fluorescent detector | Photomultiplier (2 channels),
fluorescent detector Fluorescence wavelength can be selected with the dedicated filter cube (replaceable) Exclusive equipment for the BX61WI upright microscope |
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| Transmitted DIC unit |
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| Z-drive |
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| Microscope | Upright microscopes: BX61WI, BX61 Inverted microscope: IX83 | ||||
| System control |
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| Required installation environment | Room temperature: 20 – 25°C, humidity: 75% or less@25°C, requires continuous (24-hour) power supply | ||||
| Vibration isolation table for microscope and laser installation, size | 1500mm x 1250mm |
1500 mm x1500 mm |
1700 mm x1700 mm |
1500mm x 1250mm |
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* All other third party marks are property of their respective owners.
OVERVIEW
- New anti-corrosive silver galvos increase multiphoton excitation efficiency by 50%.
- Supporting new Spectra Physics® Insight® laser for transmission out to 1300nm*.
- Dual simultaneous multiphoton wavelength excitation imaging available.
- High sensitivity hand-selected GaAsP detect or option with quantum efficiency up to 45%.
- Award winning 4mm and 8mm SCALEVIEW 25x MPE dedicated objectives for the deepest imaging of fixed samples.
- High speed measurements up to 101 cycles per second and 50,000 Hz readout with MMASW software.
- A less complex optical path with only two alignment points.
- Newly designed delivery system for optimal performance with use of the Spectra Physics® MaiTai® DeepSee® Laser, specifically tuned to match Olympus’ optical requirements.
- Olympus exclusive FastScan high speed IR laser tuning with Olympus version of MaiTai® lasers.
- Two or four Channel Non-descan Detectors.
- BX2 channel transmitted detector designed for high sensitivity detection for both transmitted fluorescence and SHG (on the upright platform only).
- Compact Design – one of the smallest footprints for MPE, 1500mm X 1500mm (60in X 60in).
- Visible imaging available – 405nm, 440nm, 473nm, 458nm, 488nm, 515nm, 559nm, and 635nm laser lines.
- Upright or inverted platforms.
- AOM standard.
- Dispersion Compensation standard.
Spectra Physics, Mai Tai, and Deep See are registered trademarks of Newport Corporation.
*Insight laser and routing optics are special order and only available in the United States and Canada.
