FV1200MPE BASIC

Multiphoton microscopy provides the greatest imaging depths in some of the most challenging samples, and demands highest sensitivity. With optical designs dedicated to providing the deepest penetration depths and light collection efficiency, the FV1200MPE Basic provides powerful tools for a wide range of applications. Configured with standard visible confocal capability and a range of descanned and non-descanned detector options, the MPE Basic is an ideal tool for core facilities and researchers with a broad range of research needs.

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SPECIFICATIONS

B System S System T System M System
Laser unit

IR pulsed laser with negative chirp for multiphoton excitation

•Mode-locked Ti : sapphire laser [femtosecond laser (equipped with a group velocity compensation)], laser power unit, water-cooled circulating chiller
IR pulsed laser can be controlled with the FV10-ASW (ver.2.1 or later) software
•MaiTai BB DeepSee-OL, MaiTai HP DeepSee-OL or MaiTai eHP DeepSee-OL (Spectra-Physics products)
MaiTai BB DeepSee-OL : 710nm — 990nm
MaiTai HP DeepSee-OL : 690nm — 1040nm
MaiTai eHP DeepSee-OL : 690nm — 1040nm (70 femtoseconds at a specimen plane)

Visible light laser AOTF laser combiner

LD laser: 405nm: 50mW, 440nm: 25mW, 473nm 15mW, 559nm 15mW, 635nm 20mW
AOTF laser combiner Multi Ar laser (457nm, 488nm, 515nm, Total 30mW), HeNe (G) laser (543nm, 1mW)
Modulation: Continuously adjustable via an AOTF (0.1 — 100% in 0.1% increments)
  • Operating mode: Allows laser turn-off during the retrace period
    REX: adjustment of laser power for each region, and selection of the laser and selection of the laser
    wavelength
  • Visible light laser platform with implemented AOTF system, ultra-fast intensity with individual laser
    lines, additional shutter control, Connected to scanner via single-mode fiber
  • Equipped with laser feedback mechanism to limit changes in laser light intensity over time
N/A
Single laser for visible light LD473 laser (15mW) Depending on the type of modulation: light intensity modulation, shutter control, connected to the scanner via single-mode optical fiber. N/A
Scanning Unit Scanning method Light deflection via 2 falvanometer scanning mirrors
Scanning modes

•Pixel size: 64 x 64 — 4096 x 4096 pixels Scanning speed: (pixel time): 2μs — 200μs High-speed scanning mode: 16 frames/s (256 x 256) •Dimensions: Time, Z, (wavelength) (or any combination thereof)
•Line scan: straight line (includes rotation), free line, point XY scan

Zoom size Observation position zoom with inclination width modification of galvanometer mirror: 1-50x (adjustable in 0.1x increments.
Confocal detector (The M scanner does not have a confocal detector)
  • Detector: 3 channels for fluorescence detection (photomultipliers), (The M scanner does not optional 1CH detector for expansion have a confocal detector)
  • Dichromatic mirrors for excitation, dichromatic mirrors for multiphoton excitation, dichromatic mirrors for fluorescence, emission filter
  • Infrared cut filter: using a high-performance filter
  • A filter or spectral type of fluorescence detector can be selected
  • Spectral type: Channels 1 and 2 provided with independent grating and slit
  • Selectable wavelength range: 1 – 100 nm, wavelength resolution: 2 nm, wavelength switching speed: 100 nm/ms
  • Pinhole: Single motorized pinhole, pinhole diameter: ø50 – 800μm (spectral type ø50 – 300μm), adjustable in 1 μm increments
  • Field Number: 18
N/A
Optics with infrared laser for multiphoton imaging
  • Integrates a multiphoton near-infrared pulsed laser in the scanning unit (Laser safety measures implemented)
  • Continuously variable output using AOM (1 – 100%, 0.1% increments)
Component incorporating the laser for multiphoton imaging
Main scanner for observation
ASU scanner for laser light stimulation, Main Scanner for observation: VIS laser
Incorporating 2 independent lasers for laser light stimulation/observation
M scanner for observation
Detector for multiphoton imaging Reflected light fluorescent detector Photomultiplier (2 or 4 channels), Fluorescence wavelength can be selected with teh dedicated filter cube (replaceable)
Transmitted light fluorescent detector Photomultiplier (2 channels), fluorescent detector Fluorescence wavelength can be selected with the dedicated filter cube (replaceable)
Exclusive equipment for the BX61WI upright microscope
Transmitted DIC unit
  • Integrated transmitted light detector and transmitted illuminator, Motorized switching
    Connected to microscope via fiber cable (IR-DIC observation using an infrared laser is not possible)
Z-drive
  • A motorized focus module inside the microscope is used
  • Minimum increment: 0.01μm
Microscope Upright microscopes: BX61WI, BX61 Inverted microscope: IX83
System control
  • OS: WindowsXP Professional (English version), WindowsVista (English version)
  • CPU: Core2Duo 2.33GHz •Memory: 2.0GB or larger
  • Hard disk: 320GB or larger •Dedicated I/F board: built-in PC •Graphics board: Open GL-compliant
  • Recording media: Equipped with DVD dual drive
Required installation environment Room temperature: 20 – 25°C, humidity: 75% or less@25°C, requires continuous (24-hour) power supply
Vibration isolation table for microscope and laser installation, size
1500mm x 1250mm
1500 mm x1500 mm
1700 mm x1700 mm
1500mm x 1250mm

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