Specifications

The FV1200MPE Exclusive is an optimized multiphoton only system. With a simplified scanhead with gold galvanometer mirrors for the highest multiphoton excitation efficiency, and high sensitivity GaAsP detectors, the FV1200MPE Exclusive provides a dedicated instrument for deep imaging in fixed and living tissue. Now supporting the new Insight laser from Spectra Physics and options such as the award winning SCALEVIEW objectives, reach the greatest imaging depths up to 8mm in SCALEVIEW-A2 cleared fixed tissue. High sensitivity detection and new high speed measurement software makes the system highly robust for applications from electrophysiology to large volume connectomics analysis.

		B System	S System	T System	M System
Laser unit	IR pulsed laser with negative chirp for multiphoton excitation	•Mode-locked Ti : sapphire laser [femtosecond laser (equipped with a group velocity compensation)], laser power unit, water-cooled circulating chiller IR pulsed laser can be controlled with the FV10-ASW (ver.2.1 or later) software •MaiTai BB DeepSee-OL, MaiTai HP DeepSee-OL or MaiTai eHP DeepSee-OL (Spectra-Physics products) MaiTai BB DeepSee-OL : 710nm — 990nm MaiTai HP DeepSee-OL : 690nm — 1040nm MaiTai eHP DeepSee-OL : 690nm — 1040nm (70 femtoseconds at a specimen plane)
	Visible light laser AOTF laser combiner	LD laser: 405nm: 50mW, 440nm: 25mW, 473nm 15mW, 559nm 15mW, 635nm 20mW AOTF laser combiner Multi Ar laser (457nm, 488nm, 515nm, Total 30mW), HeNe (G) laser (543nm, 1mW) Modulation: Continuously adjustable via an AOTF (0.1 — 100% in 0.1% increments) Operating mode: Allows laser turn-off during the retrace period REX: adjustment of laser power for each region, and selection of the laser and selection of the laser wavelength Visible light laser platform with implemented AOTF system, ultra-fast intensity with individual laser lines, additional shutter control, Connected to scanner via single-mode fiber Equipped with laser feedback mechanism to limit changes in laser light intensity over time			N/A
	Single laser for visible light	LD473 laser (15mW) Depending on the type of modulation: light intensity modulation, shutter control, connected to the scanner via single-mode optical fiber.			N/A
Scanning Unit	Scanning method	Light deflection via 2 falvanometer scanning mirrors
	Scanning modes	•Pixel size: 64 x 64 — 4096 x 4096 pixels Scanning speed: (pixel time): 2μs — 200μs High-speed scanning mode: 16 frames/s (256 x 256) •Dimensions: Time, Z, (wavelength) (or any combination thereof) •Line scan: straight line (includes rotation), free line, point XY scan
	Zoom size	Observation position zoom with inclination width modification of galvanometer mirror: 1-50x (adjustable in 0.1x increments.
	Confocal detector (The M scanner does not have a confocal detector)	Detector: 3 channels for fluorescence detection (photomultipliers), (The M scanner does not optional 1CH detector for expansion have a confocal detector) Dichromatic mirrors for excitation, dichromatic mirrors for multiphoton excitation, dichromatic mirrors for fluorescence, emission filter Infrared cut filter: using a high-performance filter A filter or spectral type of fluorescence detector can be selected Spectral type: Channels 1 and 2 provided with independent grating and slit Selectable wavelength range: 1 – 100 nm, wavelength resolution: 2 nm, wavelength switching speed: 100 nm/ms Pinhole: Single motorized pinhole, pinhole diameter: ø50 – 800μm (spectral type ø50 – 300μm), adjustable in 1 μm increments Field Number: 18			N/A
Optics with infrared laser for multiphoton imaging		Integrates a multiphoton near-infrared pulsed laser in the scanning unit (Laser safety measures implemented) Continuously variable output using AOM (1 – 100%, 0.1% increments)
Component incorporating the laser for multiphoton imaging		Main scanner for observation	ASU scanner for laser light stimulation, Main Scanner for observation: VIS laser	Incorporating 2 independent lasers for laser light stimulation/observation	M scanner for observation
Detector for multiphoton imaging	Reflected light fluorescent detector	Photomultiplier (2 or 4 channels), Fluorescence wavelength can be selected with teh dedicated filter cube (replaceable)
	Transmitted light fluorescent detector	Photomultiplier (2 channels), fluorescent detector Fluorescence wavelength can be selected with the dedicated filter cube (replaceable) Exclusive equipment for the BX61WI upright microscope
Transmitted DIC unit		Integrated transmitted light detector and transmitted illuminator, Motorized switching Connected to microscope via fiber cable (IR-DIC observation using an infrared laser is not possible)
Z-drive		A motorized focus module inside the microscope is used Minimum increment: 0.01μm
Microscope		Upright microscopes: BX61WI, BX61 Inverted microscope: IX83
System control		OS: WindowsXP Professional (English version), WindowsVista (English version) CPU: Core2Duo 2.33GHz •Memory: 2.0GB or larger Hard disk: 320GB or larger •Dedicated I/F board: built-in PC •Graphics board: Open GL-compliant Recording media: Equipped with DVD dual drive
Required installation environment		Room temperature: 20 – 25°C, humidity: 75% or less@25°C, requires continuous (24-hour) power supply
Vibration isolation table for microscope and laser installation, size		1500mm x 1250mm	1500 mm x1500 mm	1700 mm x1700 mm	1500mm x 1250mm

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SPECIFICATIONS

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