The FV10i-DOC and FV10i-LIV are the next generation of the FV10i. Its compact design can fit where you work, on a desktop or lab bench, without the need for a dedicated darkroom, bringing the power and clarity of confocal imaging to your side. Designed with ease-of-use in mind, the software has a navigator to guide even novice users to high quality data acquisition. With a powerful set of standard capabilities, the value for your research is clear.
FLUOVIEW FV10i SPECIFICATIONS
| FV10i-LIV | FV10i-DOC | |||
| Laser Light Source | Ultraviolet/Visible light LD lasers | 405nm (18mW), 473nm (12.5mW), 635nm (10mW), 559nm | ||
| Modulation: | Continuously variable by the LD direct modulation (0.1%-100%, 0.1% inclement) Line return period-laser OFF | |||
| Scanning | Scanning method | 2 galvanometer scanning mirrors | ||
| Scanning mode | Pixel size: 256 × 256 - 1024 × 1024 Scanning speed: 1.1 s / frame (for pixel size 512 × 512, High Speed scanning mode) Focusing scanning: High frame rate scan by Y- direction interlace scanning (×1, ×2, ×4) Dimension: XYT, XYZ, XYZT Rotation scanning: 0-359.9° in 0.1° increments | |||
| Detection | Detector module | Fluorescence: 2 channels, Phase Contrast: 1 channel Variable barrier filter mechanism for fluorescence channel by diffraction grating and slit | ||
| Detection method | Analog integration detection by Photomultiplier | |||
| Pinhole | Single motorized pinhole. Pinhole diameter: ø50-800μm automatic setting (adjustable to ×1.0, ×1.5, ×2.0, and ×2.5) | |||
| Field number | 18 | |||
| Optical zoom | 10× objective: 1× – 6× in 0.1x increments 60× objective: 1× – 10× in 0.1x increments |
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| Automatic Exposure | Automatic setting of the laser intensity and photomultiplier sensitivity to fluorescence intensity. | |||
| Focus | Z-drive | Motorized focus with minimum increment: 0.01μm | ||
| Objectives | Exclusively designed 10× phase contrast objective NA 0.4 (equivalent to UPLSAPO 10x) Exclusively designed 60× phase contrast water-immersion objective NA 1.2 (equivalent to UPLSAPO 60× W) Remote switching from software by electric revolver |
Exclusively designed 10× phase contrast objective NA 0.4 (equivalent to UPLSAPO 10x) Exclusively designed 60× phase contrast oil-immersion objective NA 1.35 (equivalent to UPLSAPO 60× O) Remote switching from software by electric revolver |
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| Automatic focus (AF) | Automatic detection of cover glass thickness and automatic setting of motorized correction collar. Automatic detection of interface between specimen and cover glass by laser reflection light detection | Automatic detection of interface between specimen and cover glass by laser reflection light detection | ||
| Immersion medium supply | Manual oil supply As supporting mechanism, automatic moving of XY stage to oil supply position when switching to 60x |
Automatic water supply and air cleaning mechanism for 60× | ||
| XY driving method | Motorized XY stage module by stepping motor Minimum increment: 0.3μm | |||
| XY Stage | Specimen holder | Only the dedicated specimen holder can be mounted
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Only the dedicated specimen holder can be mounted
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| Room environment: | Temperature: 37+0.1°C,0.5° C (can be switched off) Humidity: more than 90% CO2 concentration: 5% (recommended), 1 – joint fitting (ø2mm) for exterior CO2 adjustor |
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| Heating method | Noncontact heating by resistive heater mounted on frame | |||
| Controller | Dedicated controller PC/AT-compatible OS: Windows Vista Business, 32 bit (English version), CPU: Intel Core2Duo 3.0GHz RAM: 2GB × 2, HDD: 320GB × 2, Special PCI-Express I/F board built-in, Media: DVD-Multi drive built-in | |||
| Control Device | LCD monitor | 24 inch LCD monitor x 1, WUXGA (1920x1200) | ||
| Image acquisition mode | Map image, one shot, time-lapse (XYT), Z-stack (XYZ), Z-stack time-lapse (XYZT), multi area time-lapse (Multi Area XYT), multi area Z-stack time-lapse (Multi Area XYZT) | |||
| Main Software Feature | Specimen setting | Automatic setting for fluorescence channel and laser according to dye selected from list | ||
| Map image acquisition | Automatic selection of map image of 3×3 – 9x9 fields according to 10× objective lens, and manual selection of map acquisition area | |||
| Room Environment | Multi area time-lapse | Automatic multi area time-lapse by motorized XY stage Setting for each registered point: Image size, scanning speed, cross talk reduction, pinhole diameter, rotation angle, galvano zoom, acquisition channel, laser power, PMT sensitivity, Z condition. Maximum register number: 10 items per container. Maximum interval time: one hour Maximum acquisition number of times: 3000 times per point |
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| Image acquisition area | Area designation: Whole frame, clipping square area (minimum area: 96 × 96 pixels) | |||
| Image display | Display by channel, overlaid channels display, image in progress review | |||
| Cross talk reduction | Line sequential action (2 channel), or frame sequential action (3 channel and 4 channel) | |||
| Acquisition image file type | OLYMPUS image format (OIF) | |||
| Image file type available for viewing | OLYMPUS image format (OIF, OIB), Multi-TIFF format (8/16 bit grey scale, index color, 24/32/48 bit color), JPEG, BMP, TIFF | |||
| Image editing | LUT: pseudo color setting, contrast adjustment, Comment: inputting graphic, text, scale etc., image extraction, combination | |||
| 3D image construction | 3D display: Alpha Blend method, Maximum intensity projection method, 3D animation display, free orientation of cross section display | |||
| Image processing | Various types of image filter: Median, Enhanced Edge, etc. Calculations: inter-image, arithmetic and logical operation | |||
| Image analysis | Area and perimeter measurement, time-lapse measurement, colocalization analysis | |||
| Temperature | 18-28˚C | |||
| Humidity | 30-80% (non condensing) | |||
