FluoView FV10i

The FV10i-DOC and FV10i-LIV are the next generation of the FV10i. Its compact design can fit where you work, on a desktop or lab bench, without the need for a dedicated darkroom, bringing the power and clarity of confocal imaging to your side. Designed with ease-of-use in mind, the software has a navigator to guide even novice users to high quality data acquisition. With a powerful set of standard capabilities, the value for your research is clear.
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FLUOVIEW FV10i SPECIFICATIONS

FV10i-LIV FV10i-DOC
Laser Light Source Ultraviolet/Visible light LD lasers 405nm (18mW), 473nm (12.5mW), 635nm (10mW), 559nm
Modulation: Continuously variable by the LD direct modulation (0.1%-100%, 0.1% inclement) Line return period-laser OFF
Scanning Scanning method 2 galvanometer scanning mirrors
Scanning mode Pixel size: 256 × 256 - 1024 × 1024 Scanning speed: 1.1 s / frame (for pixel size 512 × 512, High Speed scanning mode) Focusing scanning: High frame rate scan by Y- direction interlace scanning (×1, ×2, ×4) Dimension: XYT, XYZ, XYZT Rotation scanning: 0-359.9° in 0.1° increments
Detection Detector module Fluorescence: 2 channels, Phase Contrast: 1 channel Variable barrier filter mechanism for fluorescence channel by diffraction grating and slit
Detection method Analog integration detection by Photomultiplier
Pinhole Single motorized pinhole. Pinhole diameter: ø50-800μm automatic setting (adjustable to ×1.0, ×1.5, ×2.0, and ×2.5)
Field number 18
Optical zoom 10× objective: 1× – 6× in 0.1x increments
60× objective: 1× – 10× in 0.1x increments
Automatic Exposure Automatic setting of the laser intensity and photomultiplier sensitivity to fluorescence intensity.
Focus Z-drive Motorized focus with minimum increment: 0.01μm
Objectives Exclusively designed 10× phase contrast objective NA 0.4 (equivalent to UPLSAPO 10x)
Exclusively designed 60× phase contrast water-immersion objective NA 1.2 (equivalent to UPLSAPO 60× W)
Remote switching from software by electric revolver
Exclusively designed 10× phase contrast objective NA 0.4 (equivalent to UPLSAPO 10x)
Exclusively designed 60× phase contrast oil-immersion objective NA 1.35 (equivalent to UPLSAPO 60× O)
Remote switching from software by electric revolver
Automatic focus (AF) Automatic detection of cover glass thickness and automatic setting of motorized correction collar. Automatic detection of interface between specimen and cover glass by laser reflection light detection Automatic detection of interface between specimen and cover glass by laser reflection light detection
Immersion medium supply Manual oil supply
As supporting mechanism, automatic moving of XY stage to oil supply position when switching to 60x
Automatic water supply and air cleaning mechanism for 60×

XY driving method Motorized XY stage module by stepping motor Minimum increment: 0.3μm
XY Stage Specimen holder Only the dedicated specimen holder can be mounted
  • For three glass bottom dishes with 35mm diameter
  • For a single cover glass chamber with 8 wells
  • For a single glass slide or well slide with 8 wells
  • Culture pod (for a glass bottom dish with 35mm diameter)
Only the dedicated specimen holder can be mounted
  • For a single glass slide or well slide with 8 wells
  • For a single glass bottom dish with 35mm diameter
Room environment:

Temperature: 37+0.1°C,0.5°
C (can be switched off)
Humidity: more than 90%
CO2 concentration: 5% (recommended), 1 – joint fitting (ø2mm) for exterior CO2 adjustor

 
Heating method Noncontact heating by resistive heater mounted on frame  
Controller Dedicated controller PC/AT-compatible OS: Windows Vista Business, 32 bit (English version), CPU: Intel Core2Duo 3.0GHz RAM: 2GB × 2, HDD: 320GB × 2, Special PCI-Express I/F board built-in, Media: DVD-Multi drive built-in
Control Device LCD monitor 24 inch LCD monitor x 1, WUXGA (1920x1200)
Image acquisition mode Map image, one shot, time-lapse (XYT), Z-stack (XYZ), Z-stack time-lapse (XYZT), multi area time-lapse (Multi Area XYT), multi area Z-stack time-lapse (Multi Area XYZT)
Main Software Feature Specimen setting Automatic setting for fluorescence channel and laser according to dye selected from list
Map image acquisition Automatic selection of map image of 3×3 – 9x9 fields according to 10× objective lens, and manual selection of map acquisition area
Room Environment Multi area time-lapse Automatic multi area time-lapse by motorized XY stage Setting for each registered point: Image size, scanning speed, cross talk reduction, pinhole diameter, rotation angle, galvano zoom, acquisition channel, laser power, PMT sensitivity, Z condition.
Maximum register number: 10 items per container.
Maximum interval time: one hour
Maximum acquisition number of times: 3000 times per point
Image acquisition area Area designation: Whole frame, clipping square area (minimum area: 96 × 96 pixels)
Image display Display by channel, overlaid channels display, image in progress review
Cross talk reduction Line sequential action (2 channel), or frame sequential action (3 channel and 4 channel)
Acquisition image file type OLYMPUS image format (OIF)
Image file type available for viewing OLYMPUS image format (OIF, OIB), Multi-TIFF format (8/16 bit grey scale, index color, 24/32/48 bit color), JPEG, BMP, TIFF
Image editing LUT: pseudo color setting, contrast adjustment, Comment: inputting graphic, text, scale etc., image extraction, combination
3D image construction 3D display: Alpha Blend method, Maximum intensity projection method, 3D animation display, free orientation of cross section display
Image processing Various types of image filter: Median, Enhanced Edge, etc. Calculations: inter-image, arithmetic and logical operation
Image analysis Area and perimeter measurement, time-lapse measurement, colocalization analysis
Temperature 18-28˚C
Humidity 30-80% (non condensing)
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