The femtoCARS equipped FV1200MPE provides a label-free lipid imaging capability based on molecular vibrations using the Coherent Anti-Stokes Raman Scattering (CARS) microscopy method. In the Olympus design, the CARS imaging mode can be combined with two photon excitation fluorescence (TPEF) and second harmonic generation (SHG) imaging modes as a multimodal femtosecond laser imaging station. CARS is one of a number of intrinsic imaging modes requiring no extrinsic label. Because it relies on molecular vibrations rather than traditional fluorescence, there is no photobleaching.
HIGH SPEED OPTOGENETICS and UNCAGING: The freedom to choose your own path
Standard raster scanning modes are not capable of measuring high speed physiology with high signal-to-noise. Olympus’ innovative Multipoint Mapping Advanced Software (MMASW) provides a solution for the most demanding high speed measurements. Using methods similar to Random Access Scanning and Targeted Path Scanning, users have the freedom to choose their own rapid multipoint measurement or stimulation paths.
Each scanned position can be expanded to a larger area.
Silver galvanometer mirrors drive the scan, ensuring access to full field of view and multicolor scanning capability with excellent light throughput for both visible and IR lasers.
Measure cell network fluctuations at up to 101 cycles per second, with up to 50,000 Hz data output per position. High signal-to-noise integration times to capture the data you need.
Use the Mapping feature to quickly identify fluorescence fluctuation positions or areas of high signal and automatically assign positions for high speed measurements.
Map cellular responses to simultaneous stimulus with Olympus’ unique SIM scanner for optogenetics or uncaging experiments (FV1200MPE BASIC and TWIN only).
Synchronize detection with electrophysiology or other external devices.
High speed electrophysiology, calcium measurements, optogenetics and FRAP/FLIP experiments. SIM scanner stimulation synchronized with imaging ensures cell responses during or immediately following stimulation are captured as they happen.
The stimulation/imaging positions and laser wavelengths can be set separately with two independent beams.
NEW! Gallium Arsenide Phosphide (GaAsP) Detector Unit
- Reach greater imaging depths with higher quantum efficiency
- High-sensitivity detection for dim samples
- High signal-to-noise ratio
NEW! ASW Software
- Advanced ASW software 4.0 breaking the RAM barrier
- Collect and open files beyond 150GB in a single multichannel Z stack
- Use ASW montage software for large-area mosaic imaging
- Match with new SCALEVIEW reagent and XLPLN 25XSVMP objective to generate 4mm deep images at and beyond Nyquist
XLPlan N 25x MPE Water Immersion Objective and UIS2 optics are recommended.
*FV1200MPE femtoCARS is a special order product only.