cell^TIRF Motorized Multicolor TIRF

The Olympus cell^TIRF illuminator offers four motorized channels for simultaneous image capture. Intuitive software control of TIRF parameters allows instant setting and confirmation of TIRF angle and seamless transition back and forth to widefield fluorescence. With the cell^TIRF illuminator, each laser wavelength is optimally focused and each angle is individually set, allowing different wavelengths to have the same penetration depth. Users can preset calculated penetration depths for all lasers with a single mouse click; the system will individually adjust each laser’s angle to simultaneously capture TIRF information from all channels.
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Olympus cell ^TIRF Illuminator APPLICATIONS

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CA Signalling

  • STIM1 Is a Calcium Sensor Specialized for Digital Signaling. Bird GS, Hwang SY, Smyth JT, Fukushima M, Boyles RR, Putney JW Jr. Curr Biol. 2009 Sep 16. Learn More>
  • Visualizing the store-operated channel complex assembly in real time: identification of SERCA2 as a new member. Sampieri A, Zepeda A, Asanov A, Vaca L. Cell Calcium. 2009 May;45(5):439-46. Epub 2009 Mar 26. Learn More>.

  • Imaging the quantal substructure of single IP3R channel activity during Ca2+ puffs in intact mammalian cells. Smith IF, Parker I. Proc Natl Acad Sci U S A. 2009 Apr 14;106(15):6404-9. Epub 2009 Mar 30. Learn More>

  • Dynamic imaging of calcium and STIM1 in the same cell using wide-field and TIRF microscopy. Walker S, Cunniffe N, Bootman M, Roderick HL. Biotechniques. 2008 Sep;45(3):347-8. Learn More>

  • A low nicotine concentration augments vesicle motion and exocytosis triggered by K(+) depolarisation of chromaffin cells. de Diego AM, Tapia L, Alvarez RM, Mosquera M, Cortés L, López I, Gutiérrez LM, Gandía L, García AG. Eur J Pharmacol. 2008 Nov 19;598(1-3):81-6. Epub 2008 Sep 16. Learn More>

  • Localization of puff sites adjacent to the plasma membrane: functional and spatial characterization of Ca2+ signaling in SH-SY5Y cells utilizing membrane-permeant caged IP3. Smith IF, Wiltgen SM, Parker I. Cell Calcium. 2009 Jan;45(1):65-76. Epub 2008 Jul 17. Learn More>

  • Imaging a target of Ca2+ signalling: dense core granule exocytosis viewed by total internal reflection fluorescence microscopy. Ravier MA, Tsuboi T, Rutter GA. Methods. 2008 Nov;46(3):233-8. Epub 2008 Oct 12. Learn More>

  • Vesicle priming and recruitment by ubMunc13-2 are differentially regulated by calcium and calmodulin. Zikich D, Mezer A, Varoqueaux F, Sheinin A, Junge HJ, Nachliel E, Melamed R, Brose N, Gutman M, Ashery U. J Neurosci. 2008 Feb 20;28(8):1949-60. Learn More>

  • Calcium Green FlAsH as a genetically targeted small-molecule calcium indicator. Tour O, Adams SR, Kerr RA, Meijer RM, Sejnowski TJ, Tsien RW, Tsien RY. Nat Chem Biol. 2007 Jul;3(7):423-31. Epub 2007 Jun 17. Learn More>

  • Calcium sparklets regulate local and global calcium in murine arterial smooth muscle. Amberg GC, Navedo MF, Nieves-Cintrón M, Molkentin JD, Santana LF. J Physiol. 2007 Feb 15;579(Pt 1):187-201. Epub 2006 Dec 7. Learn More>

  • Exocytotic release of ATP from cultured astrocytes. Pangrsic T, Potokar M, Stenovec M, Kreft M, Fabbretti E, Nistri A, Pryazhnikov E, Khiroug L, Giniatullin R, Zorec R. J Biol Chem. 2007 Sep 28;282(39):28749-58. Epub 2007 Jul 12. Learn More>

  • T cell activation requires mitochondrial translocation to the immunological synapse. Quintana A, Schwindling C, Wenning AS, Becherer U, Rettig J, Schwarz EC, Hoth M. Proc Natl Acad Sci U S A. 2007 Sep 4;104(36):14418-23. Epub 2007 Aug 28. Learn More>

  • Measurement of Ca2+ signaling dynamics in exocrine cells with total internal reflection microscopy. Won JH, Yule DI. Am J Physiol Gastrointest Liver Physiol. 2006 Jul;291(1):G146-55. Epub 2006 Feb 16. Learn More>

  • Constitutively active L-type Ca2+ channels. Navedo MF, Amberg GC, Votaw VS, Santana LF. Proc Natl Acad Sci U S A. 2005 Aug 2;102(31):11112-7. Epub 2005 Jul 22. Learn More>

  • "Optical patch-clamping": single-channel recording by imaging Ca2+ flux through individual muscle acetylcholine receptor channels. Demuro A, Parker I. J Gen Physiol. 2005 Sep;126(3):179-92. Epub 2005 Aug 15. Learn More>

Cell Adhesion and Migration

  • One-dimensional topography underlies three-dimensional fibrillar cell migration. Doyle AD, Wang FW, Matsumoto K, Yamada KM. J Cell Biol. 2009 Feb 23;184(4):481-90. Epub 2009 Feb 16. Learn More >

  • Segregation and activation of myosin IIB creates a rear in migrating cells. Vicente-Manzanares M, Koach MA, Whitmore L, Lamers ML, Horwitz AF. J Cell Biol. 2008 Nov 3;183(3):543-54. Epub 2008 Oct 27. Learn More >

  • Protein kinase C theta regulates stability of the peripheral adhesion ring junction and contributes to the sensitivity of target cell lysis by CTL. Beal AM, Anikeeva N, Varma R, Cameron TO, Norris PJ, Dustin ML, Sykulev Y. J Immunol. 2008 Oct 1;181(7):4815-24. Learn More>

  • Cell spreading and focal adhesion dynamics are regulated by spacing of integrin ligands. Cavalcanti-Adam EA, Volberg T, Micoulet A, Kessler H, Geiger B, Spatz JP. Biophys J. 2007 Apr 15;92(8):2964-74. Epub 2007 Feb 2. Learn More >

  • Regulation of protrusion, adhesion dynamics, and polarity by myosins IIA and IIB in migrating cells. Vicente-Manzanares M, Zareno J, Whitmore L, Choi CK, Horwitz AF. J Cell Biol. 2007 Feb 26;176(5):573-80. Epub 2007 Feb 20. Erratum in: J Cell Biol. 2007 Mar 26;176(7):1073. Learn More >

  • Fabrication of a dual substrate display to test roles of cell adhesion proteins in vesicle targeting to plasma membrane domains. Hunt SJ, Nelson WJ. FEBS Lett. 2007 Sep 18;581(23):4539-43. Epub 2007 Aug 27. Learn More>

  • Neural cell adhesion molecule regulates the cellular response to fibroblast growth factor. Francavilla C, Loeffler S, Piccini D, Kren A, Christofori G, Cavallaro U. J Cell Sci. 2007 Dec 15;120(Pt 24):4388-94. Epub 2007 Nov 27. Learn More>

  • Paxillin phosphorylation at Ser273 localizes a GIT1-PIX-PAK complex and regulates adhesion and protrusion dynamics. Nayal A, Webb DJ, Brown CM, Schaefer EM, Vicente-Manzanares M, Horwitz AR. J Cell Biol. 2006 May 22;173(4):587-9. Learn More>

  • Nonmuscle myosin IIA-dependent force inhibits cell spreading and drives F-actin flow. Cai Y, Biais N, Giannone G, Tanase M, Jiang G, Hofman JM, Wiggins CH, Silberzan P, Buguin A, Ladoux B, Sheetz MP. Biophys J. 2006 Nov 15;91(10):3907-20. Epub 2006 Aug 18. Learn More>

  • Syndecan-4 clustering induces cell migration in a PDZ-dependent manner. Tkachenko E, Elfenbein A, Tirziu D, Simons M. Circ Res. 2006 Jun 9;98(11):1398-404. Epub 2006 May 4. Learn More>

  • Dynamic study of the transition from hyaluronan- to integrin-mediated adhesion in chondrocytes. Cohen M, Kam Z, Addadi L, Geiger B. EMBO J. 2006 Jan 25;25(2):302-11. Epub 2006 Jan 12. Learn More>

  • Investigation of cell adhesion to structured surfaces using total internal reflection fluorescence and confocal laser scanning microscopy. Joos U, Biskup T, Ernst O, Westphal I, Gherasim C, Schmidt R, Edinger K, Pilarczyk G, Duschl C. Eur J Cell Biol. 2006 Apr;85(3-4):225-8. Epub 2005 Sep 12. Learn More>

  • Probing the integrin-actin linkage using high-resolution protein velocity mapping. Brown CM, Hebert B, Kolin DL, Zareno J, Whitmore L, Horwitz AR, Wiseman PW. J Cell Sci. 2006 Dec 15;119(Pt 24):5204-14. Learn More>

  • The glycosphingolipid, lactosylceramide, regulates beta1-integrin clustering and endocytosis. Sharma DIK, Brown JC, Cheng Z, Holicky EL, Marks DL, Pagano RE. Cancer Res. 2005 Sep 15;65(18):8233-41. Learn More>

  • Cortactin is necessary for E-cadherin-mediated contact formation and actin reorganization. Helwani FM, Kovacs EM, Paterson AD, Verma S, Ali RG, Fanning AS, Weed SA, Yap AS. J Cell Biol. 2004 Mar 15;164(6):899-910. Learn More>

Cell Membranes and Transport

  • Curved EFC/F-BAR-domain dimers are joined end to end into a filament for membrane invagination in endocytosis. Shimada A, Niwa H, Tsujita K, Suetsugu S, Nitta K, Hanawa-Suetsugu K, Akasaka R, Nishino Y, Toyama M, Chen L, Liu ZJ, Wang BC, Yamamoto M, Terada T, Miyazawa A, Tanaka A, Sugano S, Shirouzu M, Nagayama K, Takenawa T, Yokoyama S. Cell. 2007 May 18;129(4):761-72. Learn More>

  • Bi-directional transport of GLUT4 vesicles near the plasma membrane of primary rat adipocytes. Xu YK, Xu KD, Li JY, Feng LQ, Lang D, Zheng XX. Biochem Biophys Res Commun. 2007 Jul 20;359(1):121-8. Epub 2007 May 22. Learn More>
  • Direct interorganellar transfer of iron from endosome to mitochondrion. Sheftel AD, Zhang AS, Brown C, Shirihai OS, Ponka P. Blood. 2007 Jul 1;110(1):125-32. Epub 2007 Mar 21. Learn More>

  • The conserved isoleucine-valine-phenylalanine motif couples activation state and endocytic functions of beta-arrestins. Burtey A, Schmid EM, Ford MG, Rappoport JZ, Scott MG, Marullo S, Simon SM, McMahon HT, Benmerah A. Traffic. 2007 Jul;8(7):914-31. Epub 2007 Jun 4. Learn More>

  • Processive movement of single kinesins on crowded microtubules visualized using quantum dots. Seitz A, Surrey T. EMBO J. 2006 Jan 25;25(2):267-77. Epub 2006 Jan 12. Learn More>

  • Lymphocyte transcellular migration occurs through recruitment of endothelial ICAM-1 to caveola- and F-actin-rich domains. Millán J, Hewlett L, Glyn M, Toomre D, Clark P, Ridley AJ. Nat Cell Biol. 2006 Feb;8(2):113-23. Epub 2006 Jan 22. Learn More>

  • Distinct mechanisms of clathrin-independent endocytosis have unique sphingolipid requirements. Cheng ZJ, Singh RD, Sharma DK, Holicky EL, Hanada K, Marks DL, Pagano RE. Mol Biol Cell. 2006 Jul;17(7):3197-210. Epub 2006 May 3. Learn More>

  • Dynamics of clathrin and adaptor proteins during endocytosis. Rappoport JZ, Kemal S, Benmerah A, Simon SM. Am J Physiol Cell Physiol. 2006 Nov;291(5):C1072-81. Learn More>

  • In vivo selective cytoskeleton dynamics quantification in interphase cells induced by pulsed ultraviolet laser nanosurgery. Colombelli J, Reynaud EG, Rietdorf J, Pepperkok R, Stelzer EH. Traffic. 2005 Dec;6(12):1093-102. Learn More>
  • Analysis of the AP-2 adaptor complex and cargo during clathrin-mediated endocytosis. Rappoport JZ, Benmerah A, Simon SM. Traffic. 2005 Jul;6(7):539-47. Learn More>

  • Insertional assembly of actin filament barbed ends in association with formins produces piconewton forces. Kovar DR, Pollard TD. Proc Natl Acad Sci U S A. 2004 Oct 12;101(41):14725-30. Epub 2004 Sep 17. Learn More>

  • Direct imaging shows that insulin granule exocytosis occurs by complete vesicle fusion. Ma L, Bindokas VP, Kuznetsov A, Rhodes C, Hays L, Edwardson JM, Ueda K, Steiner DF, Philipson LH. Proc Natl Acad Sci U S A. 2004 Jun 22;101(25):9266-71. Epub 2004 Jun 14. Learn More>

  • Imaging single membrane fusion events mediated by SNARE proteins. Fix M, Melia TJ, Jaiswal JK, Rappoport JZ, You D, Söllner TH, Rothman JE, Simon SM. Proc Natl Acad Sci U S A. 2004 May 11;101(19):7311-6. Epub 2004 May 3. Learn More>

  • Understanding living clathrin-coated pits. Rappoport JZ, Simon SM, Benmerah A. Traffic. 2004 May;5(5):327-37. Review. Learn More>

  • Role of microtubules in fusion of post-Golgi vesicles to the plasma membrane. Schmoranzer J, Simon SM. Mol Biol Cell. 2003 Apr;14(4):1558-69. Learn More>

  • Movement of plasma-membrane-associated clathrin spots along the microtubule cytoskeleton. Rappoport JZ, Taha BW, Simon SM. Traffic. 2003 Jul;4(7):460-7. Learn More>

  • Three-dimensional analysis of post-Golgi carrier exocytosis in epithelial cells. Kreitzer G, Schmoranzer J, Low SH, Li X, Gan Y, Weimbs T, Simon SM, Rodriguez-Boulan E. Nat Cell Biol. 2003 Feb;5(2):126-36. Learn More>

  • Real-time analysis of clathrin-mediated endocytosis during cell migration. Rappoport JZ, Simon SM. J Cell Sci. 2003 Mar 1;116(Pt 5):847-55. Erratum in: J Cell Sci. 2003 Mar 15;116(Pt 6):1151. Learn More>

  • The AP-2 complex is excluded from the dynamic population of plasma membrane-associated clathrin. Rappoport JZ, Taha BW, Lemeer S, Benmerah A, Simon SM. J Biol Chem. 2003 Nov 28;278(48):47357-60. Epub 2003 Oct 6. Learn More>

  • Migrating fibroblasts perform polarized, microtubule-dependent exocytosis towards the leading edge. Schmoranzer J, Kreitzer G, Simon SM. J Cell Sci. 2003 Nov 15;116(Pt 22):4513-9. Learn More>

  • Membrane proximal lysosomes are the major vesicles responsible for calcium-dependent exocytosis in nonsecretory cells. Jaiswal JK, Andrews NW, Simon SM. J Cell Biol. 2002 Nov 25;159(4):625-35. Epub 2002 Nov 18. Learn More>

  • Insulin-regulated release from the endosomal recycling compartment is regulated by budding of specialized vesicles. Lampson MA, Schmoranzer J, Zeigerer A, Simon SM, McGraw TE. Mol Biol Cell. 2001 Nov;12(11):3489-501. Learn More>

  • Imaging constitutive exocytosis with total internal reflection fluorescence microscopy. Schmoranzer J, Goulian M, Axelrod D, Simon SM. J Cell Biol. 2000 Apr 3;149(1):23-32. Learn More>

Cell Motility

  • A tripartite complex containing MRCK modulates lamellar actomyosin retrograde flow. Tan I, Yong J, Dong JM, Lim L, Leung T. Cell. 2008 Oct 3;135(1):123-36. Learn More>

  • The cytokinesis formins from the nematode worm and fission yeast differentially mediate actin filament assembly. Neidt EM, Skau CT, Kovar DR. J Biol Chem. 2008 Aug 29;283(35):23872-83. Epub 2008 Jun 23. Learn More>

  • Lamellipodial actin mechanically links myosin activity with adhesion-site formation. Giannone G, Dubin-Thaler BJ, Rossier O, Cai Y, Chaga O, Jiang G, Beaver W, Döbereiner HG, Freund Y, Borisy G, Sheetz MP. Cell. 2007 Feb 9;128(3):561-75. Learn More>


  • A novel mitosis-specific dynamic actin structure in Dictyostelium cells. Itoh G, Yumura S. J Cell Sci. 2007 Dec 15;120(Pt 24):4302-9. Epub 2007 Nov 20.Learn More>

Cell Signaling

  • F-BAR-containing adaptor CIP4 localizes to early endosomes and regulates Epidermal Growth Factor Receptor trafficking and downregulation. Hu J, Troglio F, Mukhopadhyay A, Everingham S, Kwok E, Scita G, Craig AW. Cell Signal. 2009 Nov;21(11):1686-97. Epub 2009 Jul 24.Learn More>

  • A phosphoinositide switch controls the maturation and signaling properties of APPL endosomes. Zoncu R, Perera RM, Balkin DM, Pirruccello M, Toomre D, De Camilli P. Cell. 2009 Mar 20;136(6):1110-21. Learn More>

  • Unique secretory dynamics of tissue plasminogen activator and its modulation by plasminogen activator inhibitor-1 in vascular endothelial cells. Suzuki Y, Mogami H, Ihara H, Urano T. Blood. 2009 Jan 8;113(2):470-8. Epub 2008 Oct 15. Learn More>

  • Ci-VSP is a depolarization-activated phosphatidylinositol-4,5-bisphosphate and phosphatidylinositol-3,4,5-trisphosphate 5''''-phosphatase. Halaszovich CR, Schreiber DN, Oliver D. J Biol Chem. 2009 Jan 23;284(4):2106-13. Epub 2008 Dec 1. Learn More>

  • Identification of G protein alpha subunit-palmitoylating enzyme. Tsutsumi R, Fukata Y, Noritake J, Iwanaga T, Perez F, Fukata M. Mol Cell Biol. 2009 Jan;29(2):435-47. Epub 2008 Nov 10. Learn More>

  • Deficiency of the INCL protein Ppt1 results in changes in ectopic F1-ATP synthase and altered cholesterol metabolism. Lyly A, Marjavaara SK, Kyttälä A, Uusi-Rauva K, Luiro K, Kopra O, Martinez LO, Tanhuanpää K, Kalkkinen N, Suomalainen A, Jauhiainen M, Jalanko A. Hum Mol Genet. 2008 May 15;17(10):1406-17. Epub 2008 Feb 1. Learn More>

  • Lipid rafts determine clustering of STIM1 in endoplasmic reticulum-plasma membrane junctions and regulation of store-operated Ca2+ entry (SOCE). Pani B, Ong HL, Liu X, Rauser K, Ambudkar IS, Singh BB. J Biol Chem. 2008 Jun 20;283(25):17333-40. Epub 2008 Apr 22. Learn More>

  • Lateral diffusion of TGF-beta type I receptor studied by single-molecule imaging. Ma X, Wang Q, Jiang Y, Xiao Z, Fang X, Chen YG. Biochem Biophys Res Commun. 2007 Apr 27;356(1):67-71. Epub 2007 Feb 27. Learn More>

  • NHERF-1 and the cytoskeleton regulate the traffic and membrane dynamics of G protein-coupled receptors. Wheeler D, Sneddon WB, Wang B, Friedman PA, Romero G. J Biol Chem. 2007 Aug 24;282(34):25076-87. Epub 2007 Jun 27. Learn More>

  • LAT and NTAL mediate immunoglobulin E-induced sustained extracellular signal-regulated kinase activation critical for mast cell survival. Yamasaki S, Ishikawa E, Sakuma M, Kanagawa O, Cheng AM, Malissen B, Saito T. Mol Cell Biol. 2007 Jun;27(12):4406-15. Epub 2007 Apr 9. Learn More>

  • Surface clustering of metabotropic glutamate receptor 1 induced by long Homer proteins. Kammermeier PJ. BMC Neurosci. 2006 Jan 4;7:1. Learn More>

  • Dissociation of regulated trafficking of TRPC3 channels to the plasma membrane from their activation by phospholipase C. Smyth JT, Lemonnier L, Vazquez G, Bird GS, Putney JW Jr. J Biol Chem. 2006 Apr 28;281(17):11712-20. Epub 2006 Mar 7. Learn More>

  • Ras and its signals diffuse through the cell on randomly moving nanoparticles. Rotblat B, Yizhar O, Haklai R, Ashery U, Kloog Y. Cancer Res. 2006 Feb 15;66(4):1974-81. Learn More>

  • Matrix elasticity directs stem cell lineage specification. Engler AJ, Sen S, Sweeney HL, Discher DE. Cell. 2006 Aug 25;126(4):677-89. Learn More>

  • T cell receptor-proximal signals are sustained in peripheral microclusters and terminated in the central supramolecular activation cluster. Varma R, Campi G, Yokosuka T, Saito T, Dustin ML. Immunity. 2006 Jul;25(1):117-27. Learn More>

  • CLASP1 and CLASP2 bind to EB1 and regulate microtubule plus-end dynamics at the cell cortex. Mimori-Kiyosue Y, Grigoriev I, Lansbergen G, Sasaki H, Matsui C, Severin F, Galjart N, Grosveld F, Vorobjev I, Tsukita S, Akhmanova A. J Cell Biol. 2005 Jan 3;168(1):141-53. Learn More>

  • An angstrom scale interaction between plasma membrane ATP-gated P2X2 and alpha4beta2 nicotinic channels measured with fluorescence resonance energy transfer and total internal reflection fluorescence microscopy. Khakh BS, Fisher JA, Nashmi R, Bowser DN, Lester HA. J Neurosci. 2005 Jul 20;25(29):6911-20. Learn More>

  • Substrate binding stoichiometry and kinetics of the norepinephrine transporter. Schwartz JW, Novarino G, Piston DW, DeFelice LJ. J Biol Chem. 2005 May 13;280(19):19177-84. Epub 2005 Mar 9. Learn More>

  • JNK phosphorylates paxillin and regulates cell migration. Huang C, Rajfur Z, Borchers C, Schaller MD, Jacobson K. Nature. 2003 Jul 10;424(6945):219-23. Learn More>

Cell Transport

  • Obstacles on the microtubule reduce the processivity of Kinesin-1 in a minimal in vitro system and in cell extract. Telley IA, Bieling P, Surrey T. Biophys J. 2009 Apr 22;96(8):3341-53. Learn More>

  • Synaptotagmin VII restricts fusion pore expansion during lysosomal exocytosis. Jaiswal JK, Chakrabarti S, Andrews NW, Simon SM. PLoS Biol. 2004 Aug;2(8):E233. Epub 2004 Jun 29. Learn More>

DNA synthesis

  • Real-time single-molecule observation of rolling-circle DNA replication. Tanner NA, Loparo JJ, Hamdan SM, Jergic S, Dixon NE, van Oijen AM. Nucleic Acids Res. 2009 Mar;37(4):e27. Epub 2009 Jan 20. Learn More>


  • Quantifying exocytosis by combination of membrane capacitance measurements and total internal reflection fluorescence microscopy in chromaffin cells. Becherer U, Pasche M, Nofal S, Hof D, Matti U, Rettig J. PLoS One. 2007 Jun 6;2(6):e505. Learn More>

Gene Therapy

  • Dexamethasone-conjugated low molecular weight polyethylenimine as a nucleus-targeting lipopolymer gene carrier. Mi Bae Y, Choi H, Lee S, Ho Kang S, Tae Kim Y, Nam K, Sang Park J, Lee M, Sig Choi J. Bioconjug Chem. 2007 Nov-Dec;18(6):2029-36. Epub 2007 Sep 13. Learn More>

Lipid Bilayer Formation

  • Phospholipase D1 production of phosphatidic acid at the plasma membrane promotes exocytosis of large dense-core granules at a late stage. Zeniou-Meyer M, Zabari N, Ashery U, Chasserot-Golaz S, Haeberlé AM, Demais V, Bailly Y, Gottfried I, Nakanishi H, Neiman AM, Du G, Frohman MA, Bader MF, Vitale N. J Biol Chem. 2007 Jul 27;282(30):21746-57. Epub 2007 May 31. Learn More>
  • Phospholipid vesicle fusion on micropatterned polymeric bilayer substrates. Okazaki T, Morigaki K, Taguchi T. Biophys J. 2006 Sep 1;91(5):1757-66. Epub 2006 Jun 9. Learn More>
  • The leading edge is a lipid diffusion barrier. Weisswange I, Bretschneider T, Anderson KI. J Cell Sci. 2005 Oct 1;118(Pt 19):4375-80. Epub 2005 Sep 6. Learn More>

Microscopy Technique

  • Subdiffraction-resolution fluorescence imaging of proteins in the mitochondrial inner membrane with photoswitchable fluorophores. van de Linde S, Sauer M, Heilemann M. J Struct Biol. 2008 Dec;164(3):250-4. Epub 2008 Aug 26. Learn More>

  • Molecular dynamics and forces of a motile cell simultaneously visualized by TIRF and force microscopies. Iwadate Y, Yumura S. Biotechniques. 2008 May;44(6):739-50. Learn More>

  • Decreasing photobleaching by silver island films: application to muscle. Muthu P, Gryczynski I, Gryczynski Z, Talent J, Akopova I, Jain K, Borejdo J. Anal Biochem. 2007 Jul 15;366(2):228-36. Epub 2007 Apr 12. Learn More>

  • Two-dimensional standing wave total internal reflection fluorescence microscopy: superresolution imaging of single molecular and biological specimens. Chung E, Kim D, Cui Y, Kim YH, So PT. Biophys J. 2007 Sep 1;93(5):1747-57. Epub 2007 May 4. Learn More>

  • Single molecule high-resolution colocalization of Cy3 and Cy5 attached to macromolecules measures intramolecular distances through time. Churchman LS, Okten Z, Rock RS, Dawson JF, Spudich JA. Proc Natl Acad Sci U S A. 2005 Feb 1;102(5):1419-23. Epub 2005 Jan 24. Learn More>

  • Total internal reflection fluorescence microscopy in cell biology. Axelrod D. Traffic. 2001 Nov;2(11):764-74. Review. Learn More>


  • Imaging synaptosomal calcium concentration microdomains and vesicle fusion by using total internal reflection fluorescent microscopy. Serulle Y, Sugimori M, Llinás RR. Proc Natl Acad Sci U S A. 2007 Jan 30;104(5):1697-702. Epub 2007 Jan 22. Learn More>

RNA Structure

  • RNA folding dynamics by single-molecule fluorescence resonance energy transfer. Zhao R, Rueda D. Methods. 2009 Oct;49(2):112-7. Epub 2009 May 4. Learn More>


  • Tissue plasminogen activator (t-PA) promotes neutrophil degranulation and MMP-9 release. Cuadrado E, Ortega L, Hernández-Guillamon M, Penalba A, Fernández-Cadenas I, Rosell A, Montaner J. J Leukoc Biol. 2008 Jul;84(1):207-14. Epub 2008 Apr 7. Learn More>

Vesicle formation

  • Kiss-and-run exocytosis and fusion pores of secretory vesicles in human beta-cells. Hanna ST, Pigeau GM, Galvanovskis J, Clark A, Rorsman P, MacDonald PE. Pflugers Arch. 2009 Apr;457(6):1343-50. Epub 2008 Sep 16. Learn More>

  • Sorting of EGF and transferrin at the plasma membrane and by cargo-specific signaling to EEA1-enriched endosomes. Leonard D, Hayakawa A, Lawe D, Lambright D, Bellve KD, Standley C, Lifshitz LM, Fogarty KE, Corvera S. J Cell Sci. 2008 Oct 15;121(Pt 20):3445-58. Epub 2008 Sep 30. Learn More>

  • Dynamics of dynamin during clathrin mediated endocytosis in PC12 cells. Rappoport JZ, Heyman KP, Kemal S, Simon SM. PLoS One. 2008 Jun 11;3(6):e2416. Learn More>

  • Resolving vesicle fusion from lysis to monitor calcium-triggered lysosomal exocytosis in astrocytes. Jaiswal JK, Fix M, Takano T, Nedergaard M, Simon SM. Proc Natl Acad Sci U S A. 2007 Aug 28;104(35):14151-6. Epub 2007 Aug 21. Learn More>

  • Plasma membrane domains specialized for clathrin-mediated endocytosis in primary cells. Bellve KD, Leonard D, Standley C, Lifshitz LM, Tuft RA, Hayakawa A, Corvera S, Fogarty KE. J Biol Chem. 2006 Jun 9;281(23):16139-46. Epub 2006 Mar 13. Learn More>

  • N-WASP deficiency impairs EGF internalization and actin assembly at clathrin-coated pits. Benesch S, Polo S, Lai FP, Anderson KI, Stradal TE, Wehland J, Rottner K. J Cell Sci. 2005 Jul 15;118(Pt 14):3103-15. Epub 2005 Jun 28. Learn More>

Vesicle tracking

  • Primed vesicles can be distinguished from docked vesicles by analyzing their mobility. Nofal S, Becherer U, Hof D, Matti U, Rettig J. J Neurosci. 2007 Feb 7;27(6):1386-95. Learn More>

  • Imaging docking and fusion of insulin granules induced by antidiabetes agents: sulfonylurea and glinide drugs preferentially mediate the fusion of newcomer, but not previously docked, insulin granules. Nagamatsu S, Ohara-Imaizumi M, Nakamichi Y, Kikuta T, Nishiwaki C. Diabetes. 2006 Oct;55(10):2819-25. Learn More>

  • ELKS, a protein structurally related to the active zone-associated protein CAST, is expressed in pancreatic beta cells and functions in insulin exocytosis: interaction of ELKS with exocytotic machinery analyzed by total internal reflection fluorescence microscopy. Ohara-Imaizumi M, Ohtsuka T, Matsushima S, Akimoto Y, Nishiwaki C, Nakamichi Y, Kikuta T, Nagai S, Kawakami H, Watanabe T, Nagamatsu S. Mol Biol Cell. 2005 Jul;16(7):3289-300. Epub 2005 May 11. Learn More>

  • Selective nucleotide-release from dense-core granules in insulin-secreting cells. Obermüller S, Lindqvist A, Karanauskaite J, Galvanovskis J, Rorsman P, Barg S. J Cell Sci. 2005 Sep 15;118(Pt 18):4271-82. Epub 2005 Sep 1. Learn More>

  • TIRF imaging of docking and fusion of single insulin granule motion in primary rat pancreatic beta-cells: different behaviour of granule motion between normal and Goto-Kakizaki diabetic rat beta-cells. Ohara-Imaizumi M, Nishiwaki C, Kikuta T, Nagai S, Nakamichi Y, Nagamatsu S. Biochem J. 2004 Jul 1;381(Pt 1):13-8. Learn More>


  • Imaging the interaction of HIV-1 genomes and Gag during assembly of individual viral particles. Jouvenet N, Simon SM, Bieniasz PD. Proc Natl Acad Sci U S A. 2009 Nov 10;106(45):19114-9. Epub 2009 Oct 27. Learn More>

  • Imaging the biogenesis of individual HIV-1 virions in live cells. Jouvenet N, Bieniasz PD, Simon SM. Nature. 2008 Jul 10;454(7201):236-40. Epub 2008 May 25. Learn More>

  • A novel supervised trajectory segmentation algorithm identifies distinct types of human adenovirus motion in host cells. Helmuth JA, Burckhardt CJ, Koumoutsakos P, Greber UF, Sbalzarini IF. J Struct Biol. 2007 Sep;159(3):347-58. Epub 2007 Apr 14. Learn More>

  • Plasma membrane is the site of productive HIV-1 particle assembly. Jouvenet N, Neil SJ, Bess C, Johnson MC, Virgen CA, Simon SM, Bieniasz PD. PLoS Biol. 2006 Dec;4(12):e435. Learn More>


Screening drugs to block aggregation of Huntingtin protein

Product Overview. Biocompare interview with Olympus Associate Product Manager, Stuart Shand

Instrument: cell^TIRF. The 5th Generation of TIRF illuminator from Olympus

Screening drugs to block aggregation of Huntingtin protein

Actin Polymerization

Instrument: Olympus IX71. 60X 1.45NA

Screening drugs to block aggregation of Huntingtin protein

FoLu cells with EGFP Vinculin

Instrument: Olympus IX81 with Zero-Drift. 100X 1.45NA

Screening drugs to block aggregation of Huntingtin protein

FoLu cells with mEmerald AlphaActinin and mCherry LifeAct

Instrument: Olympus IX81 with Zero-Drift. 100X 1.45NA

Screening drugs to block aggregation of Huntingtin protein

FoLu cells with mEmerald LifeAct and mCherry Zyxin

Instrument: Olympus IX81 with Zero-Drift. 100X 1.45NA

Screening drugs to block aggregation of Huntingtin protein

FoLu cells with mEmerald Tubulin and mApple Zyxin

Instrument: Olympus IX81 with Zero-Drift. 60X 1.45NA

Screening drugs to block aggregation of Huntingtin protein

LLC-PK1 cells with mEGFP Cadherin and mApple Cadherin

Instrument: Olympus IX81 with Zero-Drift. 100X 1.45NA

Screening drugs to block aggregation of Huntingtin protein

LLC-PK1 cells with mEGFP Cadherin and mApple Cadherin

Instrument: Olympus IX81 with Zero-Drift. 100X 1.45NA

Screening drugs to block aggregation of Huntingtin protein

LLC-PK1 cells with mEGFP Cx26 and mApple Cadherin

Instrument: Olympus IX81 with Zero-Drift. 100X 1.45NA

  • BX3 fits meDP80
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  • VS120 Slide Systemfvmpe_rs
  • Specialty Objectives Imaris by Bitplane
  • CX22cellsens Digital Imaging Software