cellSens Digital Imaging Software for Research Applications

The Olympus cellSens platform creates a uniquely personal and intuitive imaging experience based on your preferred workflow. With cellSens you have full control over the display and placement of icons, toolbars and controls to simplify your desktop and enhance your productivity.

cellSens is easy to use, powerful and flexible. Tailoring the software to your budget and imaging applications is easy with its modular design. As your research needs evolve, cellSens grows with you.
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SPECIFICATIONS



cellSens* Package ENTRY STANDARD DIMENSION

 

Image Acquisition

Image Acquisition Snapshot/Movie X X X
Time-Lapse   X X
Z-Stack     X
Automatic acquisition of multi channel Images   Multichannel Acquisition X
Automatic acquisition of multi channel Images combined with time lapse and Z-Stack acquisition     X
GEM (Graphical Experiment Manager)     X
Multiple Image Alignment Manual MIA   Manual Process X
Automatic MIA     Multi Pos.
Extended Focal Imaging Instant EFI (manual Z)   Manual Process X
EFI (Z stack)     X
Multi Position Stage Navigator and Position List     Multi Pos.
Well plate scan
Acquisition of samples in a well plate format
   

Well Nav. & Multi Pos.

Deconvolution Offline and online 2D Deconvolution     X
HDR High Dynamic Range Imaging (Qualitative)     X
Image Editing Image synthesis Multi-image alignment (MIA), color image, Color image combine, channel combine   X X
Frame combine     X
Mode selection RGB 24-/48-bit color, multi-channel, gray scale, 8-/16-bit channel X X X
Geometry Resize, mirroring, trim, rotation (90°, 180°, 270°), channel shift.   X X
Z stack adjustment, time adjustment     X
Calibration Spatial calibration   X X
Brightness calibration     X
Image Processing Logics and computations Add, subtract, multiply, divide, minimum, maximum, Boolean Operations (AND, OR, XOR)     X
Edge detection filters Laplace, Sobel, Roberts     X
Smoothing filters Low-pass   X X
Sigma, Median, Rank, NxN     X
Sharpness Sharpen, High-pass   X X
Morphological Filter

Erosion, Dilation, Morphological Open, Morphological Close, Gradient, Top Hat Bright, Top Hat Dark, Distance Bright, Distance Dark, Ultimate Erode Bright, Ultimate Erode Dark, Skeleton Bright, Skeleton Dark, Separate Objects

    X
Tone adjustment Brightness adjustment, RGB adjustment, contrast optimization, contrast maximization, shading correction, background subtraction, background correction, white balance, reversal   X X
Offline EFI, Fluorescence     X
Brightfield Unmixing     X
Fluorescence Unmixing     X
Deconvolution Nearest Neighbor, Wiener (inverse filter), Iterative 2D deconvolution     X
Constrained Iterative 3D Deconvolution     CI Decon
Projection

Max/Min/Ave of Z, Max/Min/Ave of time, Max/Min/Ave of wavelength

    X
Image Measurement Manual Measurement Origin / Length of straight and bent lines / Circumference and area of circle, ellipse, rectangle and polygon / Angle X X X
Closed polygon, Free hand, Magic wand     X
Threshold analysis

Auto thresholds, manual HSV thresholds, manual RGB thresholds.
Threshold based segmentation into phases, basic measurements on phases.

    X
Threshold based segmentation into objects, advanced measurements on objects.     C&M Full
Off-line Ratio ROI based calculation of ratios and calibrated calcium concentrations     X
Intensity profile

Methods: Over Time or Z-Stack
Results: Ave., maximum, minimum, integration

    X
Dynamic ROI's Region measurements using moving ROI's in time-lapse movies and Z-stacks     X
Colocalization Determine and measure colocalization of marker pairs..     X
Export Export to Excel (.xls)   X X
Export to Olympus Workbook (.owb)     X
Windows Layout Switching of document layout   X X
Floating/docking of document group     X
Customization User customization of toolbar and menus   X X
Reporting

Document compilation, image insertion, save, print, PDF/RTF export
No support for 64-bit MS Office

    X
Software protection Web based software protection X X X
Color space sRGB / Adobe RGB (DP72 DP73 compatible) X X X
File formats

<Save/Load>
JPEG, JPEG2000, TIFF, BMP, AVI, PNG, VSI (Virtual Slide Image), BigTIFF,
PSD(Adobe photoshop)
<Load only>
GIFF, oif, oir, oib (Fluoview format), MRC (Medical Research Council), STK (Metamorph), FSX

X X X
Image View Single Frame View/Title View   X X
Slice View     X
Voxel View     X
1: Three Point angle, four Point angle, Arbitrary Line, Closed polygon, Polyline and Perpendicular line only.

Appendix 3: cellSens 1.8 Supported Devices

cellSens Package
ENT
ST
DI
Application Cameras Olympus DP®* DP20, DP21, DP25, DP26, DP70, DP71, DP72, DP73, DP73WDR,  DP80, TWAIN I/F
X
X
X
OSIS

CC-12, Colorview I, Colorview II, Colorview III, Colorview IIIu, F-view II,
XM10, XC10, XC30, XC50, UC30, UC50, SC20, SC30, SC100

X
X
X
Hamamatsu* ORCA® (03, 05, AG, ER, R2, Flash 2.8), C9300
X

Orca Flash 4.0 V2
ImageEM (C9100-13)

High-End Camera
QImaging®*

Monochrome: Exi Blue/Aqua, RETIGA (Exi, SRV, 2000R, 2000RV, 4000R, 4000RV) QiClick plus RGB slider
Color: Exi Aqua

X
MicroPublisher 3.3 RTV/3.3 RTV cooled, MicroPublisher 5 RTV/5 RTV cooled
X
X
RoleraTM Thunder
High-End Camera
AndorTM* iXon DU897 X3, iXon DU897 Ultra
High-End Camera
Photometrics CoolSnap HQ2
X
Evolve 512 Delta
High-End Camera
Jenoptik ProgRes C3, ProgRes C5
X
X
Camera Control Camera control

Live view, image acquisition, exposure time (Manual/Auto), exposure indicator, white balance, black balance (*1), online shading correction (*2), custom gray scale (*3).
[*: Supported functions are dependent on the camera models as listed below (DP series).] *1: DP70, DP71, DP72. / *2: DP25 / *3: DP71, DP72, DP73

X
X
X

Autofocus
(motorized Z focus is necessary)

X
Display function Zooming, panning, annotation, overlay, view/hide fluorescence channel, tile view/slice view, series image animation (play, stop, forward, reverse), frame extraction.
X
X
X
Focus indicator, line profile, histogram (linear display/logarithmic display), information stamp, scale bar, memo.
X
X
X
Microscope Control Microscope fames

IX83 ®(*4), IX73 (*4), BX63®, BX53, BX43, BX51, BX61, BX61WI, IX71, IX81, IX81-ZDC/ZDC2, SZX 9, SZX 10, SZXII 12, SZXII 16, MVX10, Manual microscope, manual stereo microscope.

X
X
Motorized devices

IX3-CBH (*4), IX3-CBM*4, U-CB5S, U-CBF, BX3-CBH, BX3-CBM, BX3-SSU, BX3-SSU(RS232C),  BX2-UCB, BX-REMCB, IX2-UCB, SZX-MDCU, SZX2-MDCU, DSU, IX3-DSU, IX3-MLWCDA, IX3-SSU,ZDC1/2, IX3-ZDC, IX3-DICTA.

X
X
OSIS motorized cell^TIRF, MT20, USB-ODB converter,FCB Controller, Real Time Controller (RTC), IX- & BX-FCB (FFW, FFWEM & FFR (filter wheels), FSHU (shutter), IX2-FRACA.    
X
Devices Real Time Controller (RTC), FCB    
X
Microscope Control Non-Olympus motorized devices Shutter: Uniblitz shutter (VCM-D1, VMM-D1/D3)
X
X

XY motorized stage:
Prior (ProScan I, ProScan II, ProScan III, OptiScan, ES101EZ)
Ludl (Mac 6000)
Lang (Lstep serial, Lstep PCI)
Objective Imaging (OASIS 4i)
ITK (Corvus)
Märzhäuser (Tango)

   

Multi-position

Shutter, filter wheel and Z motor:
Prior (ProScan I, ProScan II, ProScan III, OptiScan, ES101EZ)
Ludl (Mac 6000)
Shutter and filter wheel:
Sutter (Lambda 10-3/Lambda 10-B)

Z motor:
Lang (Lstep serial, Lstep PCI)
Objective Imaging (OASIS 4i)

 
X

Fluorescence lamp:
CoolLED precisExcite , EXFO X-Cite (120PC XL, exacte, XLED1)

 
X
Device customization Observation methods, hand switch, frame, soft keys  
X
X
*If you are updating an IX3 system from cellSens 1.7/1.7.1 to 1.9, it is highly recommended to update IX3 firmware to the latest version. The updated software is provided in the install program and you can also find the link from ''''Driver Installation'''' file, which will be created on your desktop during software installation.

Appendix 4: cellSens 1.9 System Requirements

OS Windows® 8 (32-bit/64-bit) Professional with SP1
Windows® 8 (32-bit/64-bit) Ultimate with SP1
Windows® 7 (32-bit/64-bit) Ultimate with SP1
Windows® 7 (32-bit/64-bit) Professional with SP1
Limited compatability of third-party hardware to both Windows 8 Professional and Ultimate.
CPU Intel Core i5, Intel Core i7, Intel Xeon(R)
recommended for high speed image acquisition: QuadCo
RAM

4 GB
recommended for high speed image acquisition: 8 GB or more

Graphic card 1280x1024 (min. 1024 x768) monitor resolution with 32-bit-video card with separate graphics memory (no integrated graphics processor with shared memory)
Ports

USB 2.0 port to connect devices to the system
Fire Wire A to connect devices to the system (BX63, IX83 etc...)
Serial (RS232) to connect devices to the system (BX61, IX81, SZX2-MDCU etc...)

HDD Space

1 GB for installation
Performance of hard disk could be a limiting factor for image acquisition speed.
Recommended for high speed image acquisition: SSD hard disk.

Drive DVD-ROM drive (for installation)
Web Browser Recommended for Microsoft Internet Explorer 8.0.

Note that the specifications above describe the requirements for proper software function. If computer controlled hardware is also connected to the microscope, the PC specifications also need to meet its requirements.

*cellSens, DP, Hamamatsu, QImaging, Andor cameras are not for clinical diagnostic use in the United States.

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