cellSens Digital Imaging Software for Research Applications

The Olympus cellSens platform creates a uniquely personal and intuitive imaging experience based on your preferred workflow. With cellSens you have full control over the display and placement of icons, toolbars and controls to simplify your desktop and enhance your productivity.

cellSens is easy to use, powerful and flexible. Tailoring the software to your budget and imaging applications is easy with its modular design. As your research needs evolve, cellSens grows with you.
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SPECIFICATIONS



cellSens* Package EN ST DI

 

Image Acquisition

Image Acquisition Snapshot/Movie X X X
Time-Lapse   X X
Z-Stack     X
Automatic acquisition of multi channel Images   Multichannel Acquisition 5D
Automatic acquisition of multi channel Images combined with time lapse and Z-Stack acquisition     5D
Multiple Image Alignment Manual MIA   Manual Process X
Automatic MIA     Multi Pos.
Extended Focal Imaging Instant EFI (manaual Z)   Manual Process X
EFI (Z stack)     X
Multi Position Satge Navigator and Position List     Multi Pos.
Well plate scan
Definable for the well plate format
   

Well Nav. & Multi Pos.

Deconvolution Online 2D Deconvolution     X
HDR High Dynamic Range Imaging (Qualitative)     X
Image Editing Image synthesis Multi-image alignment (MIA), color image, Color image coupling, channel coupling   X X
Frame coupling     X
Mode selection RGB 24-/48-bit color, multi-channel, gray scale, 8-/16-bit channel X X X
Geometry Resize, mirroring, trimming, rotation (90°, 180°, 270°), channel shifting.   X X
Z stack adjustment, time adjustment     X
Calibration Image calibraiton   X X
Brightness calibration     X
Image Processing Logics and computations Addition, subtraction, multiplication, division, AND, OR, XOR, minimize, maximize     X
Edge detection filters Laplacian     X
Smoothing filters Low-pass   X X
Sigma, Median, Rank, NxN     X
Sharpness Sharpen, High-pass   X X
Morphological Filter

Erosion, Dilation, Morphological Open, Morphological Close, Gradient, Top Hat Bright, Top Hat Dark, Distance Bright, Distance Dark, Ultimate Erode Bright, Ultimate Erode Dark, Skeleton Bright, Skeleton Dark, Separate Objects

    X
Tone adjustment Brightness adjustment, RGB adjustment, contrast optimization, contrast maximization, shading correction, background subtraction, background correction, white balance, reversal   X X
Offline EFI, Fluorescence     X
Brightfield Unmixing     C&M Full
Fluorescence Unmixing     5D
Deconvolution Nearest Neighbor, Wiener (inverse filter), Iterative 2D deconvolution     X
Constrained Iterative 3D Deconvolution     CI Decon
Projection

Max/Min/Ave of Z, Max/Min/Ave of time, Max/Min/Ave of wavelength
ight and bent lines / Circumference

    X
Image Measurement Manual Measurement Origin / Length of straight and bent lines / Circumference and area of circle, ellipse, rectangle and polygon / Angle X X X
Closed polygon, Free hand, Magic wand     X
Threshold analysis

Auto thresholds, manual HSV thresholds, manual RGB thresholds.
Threshold based segmentation into phases, basic measurements on phases.

    X
Threshold based segmentation into objects, advanced measurements on objects.     C&M Full
Intensity profile

Methods: Over Time or Z-Stack
Results: Ave., maximum, minimum, integration

    X
Export Export to Excel (.xls)   X X
Export to Olympus Workbook (.owb)     X
Windows Layout Switching of document layout   X X
Floating/docking of document group     X
Customization User customization of toolbar and menus   X X
Reporting

Document compilation, image insertion, save, print, PDF/RTF export
(do not support 64-bit office)

    X
Software protection Web based software protection X X X
Color space sRGB / Adobe RGB (DP72 DP73 compatible) X X X
File formats

<Save/Load>
JPEG, JPEG2000, TIFF, BMP, AVI, PNG, VSI (Virtual Slide Image), BigTIFF,
PSD(Adobe photoshop)
<Load only>

GIFF, oif (Fluoview format), oib (Fluoview format), MRC (Medical Research Council), STK (Metamorph), FSX
X X X
Image View Single Frame View/Title View   X X
Slice View     X
Voxel View     5D or C&M Full
1: Three Point angle, four Point angle, Arbitrary Line, Closed polygon, Polyline and Perpendicular line only.

Appendix 3: cellSens 1.8 Supported Devices

cellSens Package
ENT
ST
DI
  Olympus DP®* DP20, DP21, DP25, DP26, DP70, DP71, DP72, DP73, DP73WDR,  DP80, TWAIN I/F
X
X
X
OSIS

CC-12, Colorview I, Colorview II, Colorview III, Colorview IIIu, F-view II,
XM10, XC10, XC30, XC50, UC30, UC50, SC20, SC30, SC100

X
X
X
Hamamatsu* ORCA® (03, 05, AG, ERG, R2, Flash 2.8), C9300
X

Orca Flash 4.0
ImageEM (C9100-13)

High-End Camera
QImaging®*

Monochrome: Exi Blue/Aqua, RETIGA (Exi, SRV, 2000R, 2000RV, 4000R, 4000RV) QiClick plus RGB slider
Color: Exi Aqua

X
MicroPublisher 3.3 RTV/3.3 RTV cooled, MicroPublisher 5 RTV/5 RTV cooled
X
X
RoleraTM Thunder
High-End Camera
AndorTM* iXon DU897 X3
High-End Camera
Photometrics CoolSnap HQ2
X
Evolve 512 Delta
High-End Camera
Jenoptik ProgRes C3, ProgRes C5
X
X
Camera Control Camera control

Live view, image acquisition, exposure time (Manual/Auto), exposure indicator, white balance, black balance (*1), online shading correction (*2), custom gray scale (*3).
[*: Supported functions are dependent on the camera models as listed below (DP series).] *1: DP70, DP71, DP72. / *2: DP25 / *3: DP71, DP72, DP73

X
X
X

Autofocus
(motorized Z focus is necessary)

X
Display function Zooming, panning, annotation, overlay, view/hide fluorescence channel, tile view/slice view, series image animation (play, stop, forward, reverse), frame extraction.
X
X
X
Focus indicator, line profile, histogram (linear display/logarithmic display), information stamp, scale bar, memo.
X
X
X
Microscope Control Microscope fames

IX83 ®(*4), IX73 (*4), BX63®, BX53, BX43, BX51, BX61, BX61WI, IX71, IX81, IX81-ZDC/ZDC2, SZX 9, SZX 10, SZXII 12, SZXII 16, MVX10, Manual microscope, manual stereo microscope.

X
X
Motorized devices

IX3-CBH (*4), IX3-CBM*4, U-CB5S, U-CBF, BX3-CBH, BX3-CBM, BX3-SSU, BX3-SSU(RS232C),  BX2-UCB, BX-REMCB, IX2-UCB, SZX-MDCU, SZX2-MDCU, DSU, IX3-DSU.

X
X
OSIS motorized cell^TIRF, MT20, USB-ODB converter.    
X
Devices Real Time Controller (RTC), FCB    
X
Microscope Control Non-Olympus motorized devices Shutter: Uniblitz shutter (VCM-D1, VMM-D1/D3)
X
X

XY motorized stage:
Prior (ProScan I, ProScan II, ProScan III, OptiScan, ES101EZ)
Ludl (Mac 6000)
Lang (Lstep serial, Lstep PCI)
Objective Imaging (OASIS 4i)
ITK (Corvus)
Märzhäuser (Tango)

   

Multi-position

Shutter, filter wheel and Z motor:
Prior (ProScan I, ProScan II, ProScan III, OptiScan, ES101EZ)
Ludl (Mac 6000)
Shutter and filter wheel:
Sutter (Lambda 10-3/Lambda 10-B)

Z motor:
Lang (Lstep serial, Lstep PCI)
Objective Imaging (OASIS 4i)

 
X

Fluorescence lamp:
CoolLED precisExcite , EXFO X-Cite (120PC XL, exacte, XLED1)

 
X
Device customization Observation methods, hand switch, frame, soft keys  
X
X

Appendix 4: cellSens 1.8 System Requirements

OS

Microsoft® Windows® Vista (32-bit) Ultimate with SP2
Microsoft® Windows® Vista (32-bit) Business with SP2
Windows® XP Professional (32-bit) with SP3
Windows® 7 (32-bit/64-bit) Ultimate with SP1
Windows® 7 (32-bit/64-bit) Professional with SP1

CPU Intel Core i5, Intel Core i7, Intel Xeon(R)
recommended for high speed image acquisition: QuadCo
RAM

4 GB
recommended for high speed image acquisition: 8 GB or more

Graphic card 1280x1024 (min. 1024 x768) monitor resolution with 32-bit-video card with separate graphics memory (no integrated graphics processor with shared memory)
Ports

USB 2.0 port to connect devices to the system
Fire Wire A to connect devices to the system (BX63, IX83 etc...)
Serial (RS232) to connect devices to the system (BX61, IX81, SZX2-MDCU etc...)

HDD Space

1 GB for installation
Performance of hard disk could be a limiting factor for image acquisition speed.
Recommended for high speed image acquisition: SSD hard disk

Drive DVD-ROM drive (for installation)
Web Browser Recommended for Microsoft Internet Explorer 8.0

Note that the specifications above describe the requirements for proper software function. If computer controlled hardware is also connected to the microscope, the PC specifications also need to meet its requirements.

*cellSens, DP, Hamamatsu, QImaging, Andor cameras are not for clinical diagnostic use in the United States.

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