Andor Revolution XD Spinning Disk Confocal

When it comes to live cell imaging, you need the best optics and a system with tight opto-electronic integration to capture those dynamic events. Olympus and Andor have built reputations for optical excellence and flexible system designs to meet the most challenging research needs. Collaboration between Olympus and Andor means outstanding support and true partnership for your research.
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Microscopy technique

Revolution XD: the superior choice for live cell imaging

  • Spinning disk confocal is ideal when laser scanning confocal speeds are insufficient.
  • Highly flexible laser combiner allows for the use of up to 6 lasers. Lasers may be added or exchanged to accommodate user needs.
  • Optical train customized to eliminate internal reflections, hence minimizing background signal.
  • Precision mounting system for optical train assures optimum alignment and flatness of field for inverted and upright microscopes.
  • Tight synchronization of Andor laser combiner with Andor time-course studies.
  • Highly configurable acquisition through MetaMorph® software.
  • High frame rates plus piezo Z capability make the Revolution XD a great source for acquiring live multi-color 3D volumes over time.


Revolution components and systems benefit from common features across applications. State of the art performance, flexible component-oriented supply and an open-minded approach to systems and technically demanding solutions. Microscopy application

Key Applications

  • Cell Motility
  • Live Cell Confocal
  • FRET Imaging
  • Ion Signalling
  • Fluorescent Proteins
  • Single Molecule
  • Comet Assay
  • FISH
  • Immunofluorescence
  • Bio/Chemiluminescence


There are a diverse group of microscopes that are compatible with the Andor system. These microscopes feature IX81® ZD2, IX71, BX61®, BX61WI and BX63.


  • Active high speed focus compensation support
  • Control microscope motorization
  • Vibration isolation tables
  • Stage top and Cage incubators
  • Options for temperature, humidity and CO2 regulation
  • Compatible with manual and fully motorized stands even the Olympus Up/Down Microscope

Camera Options

iXon3 Clara Neo
Single Photon Sensitivity Resolution & Sensitivity Drive Optimal Performance
ixon3 confocal dual spinning disk Neo


  • Single photon sensitivity and back-illuminated sensors
  • UltraVacTM cooling to -100°C
  • Unique ultrafast Crop Mode
  • OptAcquire one-click optimization
  • Count Convert calibrates in electrons or photons
  • EMCALTM self-calibration of EM Gain
  • DU-897 - The ultimate combination of sensitivity and frame rate
  • DU-888 - 1MP resolution and exceptional sensitivity combined
  • DU-860 - For high speed applications e.g Ca2+ flux

  • Features

    • Ideal for high-resolution cell microscopy
    • 1.3 megapixel interline CCD; 6.45 μm pixels
    • Very low readout noise (< 3e-)
    • Deep thermoelectric vacuum cooling down to -45°C
    • Broad Vis-NIR wavelength coverage (with enhanced NIR version available)
    • Up to 20 MHz readout speed
    • USB 2.0 connectivity


    • Lower detection limit than any CCD camera
    • 5.5 megapixel sensor format and 6.5 um pixels
    • Minimization of dark current and pixel blemishes
    • Rapid frame time rates


    Confocal Spinning Disk Unit

    Confocal spinning disk
    • 1500 - 10,000 rpm disk, producing between 300 to >2000 frames per second
    • Easy synchronization of exposure to disk speed
    • Manual and motorized filter changer models available
    • Optional bright field bypass module
    • 2x excitation throughput improvement compared to CSU10 or CSU22
    • FWHM Z section < =1.2 μm
    • Semrock dichroic and emission filter pairing provides optimum Signal to Noise Ratio (SNR)
    • Filter wheel mounting for fluorescence and DIC analyzer
    • Andor 10 position filter wheel available
    • Yokogawa 6 and 12 positions filter wheels

    Laser Combiner

    Compact, flexible, robust
    Opto-electronic control

    • Integral AOTF for microsecond laser selection and modulation
    • Hardware blanking minimizes specimen exposure
    • Long life solid state lasers with excellent stability (typical ± 0.5% peak to peak)
    • Up to 6 solid state lasers (from 405, 445, 488, 491, 515,
      532, 561, 594, 640 nm)
    • Powers from 25 - 250 mW depending on wavelength
    • Compact 4U 19” rack mount enclosure
    • Quiet, cool with low power consumption

    Precision Controller Unit

    Flexible Expansion

    Precision Controller
    • Provides system synchronization
    • PCUB - Houses AOTF driver for ALC coupling
    • Optional 16 bit Analog output for direct piezo Z control
    • 8 digital inputs and 8 digital outputs for sensing and triggering external events
    • Enables Revolution™ to slave or master for complex triggering synchronization
    • Metamorph® control

    Imaging Workstation

    Optimized PC workstation for live cell work
    Image Workstation

    Handling, processing and visualizing multidimensional images is very computer intensive. The Andor workstation is a high end Desktop PC optimized for use with Metamorph and for handling large data sets which arise from experiments that employ Andor cameras.


    • High end Windows® workstation
    • Acquisition bandwidth for dual camera support
    • ImageDisk removes dependency on RAM for huge datasets
    • Multi-core processors for multi-threaded software
    • High performance graphics with OpenGL
    • Real-time volume rendering with Bitplane Imaris option
    • Designed to run MetaMorph for Olympus Premier configured for the Andor XD

    Motorized XY & Z Control

    Z Stage XY stage

    Z Stage Features

  • Piezo stages with 100, 200 and 500 μm ranges
    1 nm repeatability
  • 2-8 nm resolution (depends on range)
  • Up to 100 Z sections/s
  • Specimen holders - slides, petri and microtitre plates

  • XY Stage Features

  • Open and closed loop stages
  • Typical travel >100 x 75 mm, with 0.02 μm resolution
  • 30 mm/sec travel speed
  • Perform multi-field scans for 6D imaging
  • Create 4D mosaics
  • Repeatability 0.2 to 0.3 μm rms

  • Piezo Objective Features

  • PI PIFOC P721 - 100 μm travel
  • PI PIFOC P725 - 400 μm travel
  • Up to 20 Z sections/s
  • 1.25 nm resolution
  • Analog or digital control
  • Oil and water objectives

  • Camera Port Adaptors

    Single & Dual Port
    Camera Port

    Single Port Coupler Features

    • Magnification X = 1.0, 1.2, 1.5, 2.0 (others by request)
    • Compatible with filter wheel or CSU+ filter wheel
    • Achromatic 450-650 nm
    • AR-coated
    • Transmission > 98%
    • Adjustable XY centering
    • Sliding barrel focus control
    Dual Port Coupler Features

  • Enables simultaneous two-camera exposure with discrimination by wave length or polarization. A 100% mirror can be included for easy camera switching.
  • Magnification X = 1.0, 1.2, 1.5, 2.0 (others by request)
  • Compatible with 'C'/'CS' lenses, C-mount, filter wheel or CSU+filter wheel
  • Magnification select in each arm
  • Mounting cassettes 'store adjustment' simplifying exchange and minimizing readjustment

  • Filter Wheels & Splitters

    Emission discrimination
    Filter Wheels


    TR-FW10 Features

    • 6,10 & 12 position, ø25 filter wheels
    • Optional support for Ludl, Prior, ASI and Yokogawa wheels
    • Matched to CSU or microscope C-mount coupler
    • Infinity optical path for optimum filter performance
    • External controller allows support of Sutter SmartShutter

    Field Splitter Features

    • Optosplit II and III which use 25 mm filters
    • Field splitting for simultaneous 2 or 3 color imaging
    • Matched to CSU or microscope C-mount coupler
    • DualViewTM and QuadView also supported

    MetaMorph is a registered trademark of MDS Analytical Technologies, Inc.
    Andor, the Andor logo and Revolution are trademarks of Andor Technology Plc.


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