CLARITY, SeeDB, SCALEVIEW

Tissue clearing with the SCALEVIEW-A2, CLARITY and SeeDB clearing techniques has opened up new possibilities for deep imaging. When coupled with clearing, Olympus Multi-Immersion objectives now allow researchers to reconstruct vast tissue areas at high resolution with the FV1200, FV1200MPE, and FVMPE-RS systems.
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All images obtained using an Olympus FluoView FV1000-MPE multiphoton microscope system with either SCALEVIEW immersion 25x MPE 4mm or 8mm objective and SCALEVIEW-A2 optical clearing agent.

Video:

Dr. Atsushi Miyawaki discusses many topics including the revolutionary Scale clearning technique allowing 4mm deep multiphoton imaging for Connectome work.

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Image of YFP-H mouse hippocampus acquired as 24 region (4x6) montage over 3.3mm of depth, and stitched using Olympus FluoView software.

Image of YFP-H mouse hippocampus acquired as 24 region (4x6) montage over 3.3mm of depth, and stitched using Olympus FluoView software.

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The image shows 4 mm deep acquisition of mouse brain transgenically encoded to express YFP in neurons.

The image shows 4 mm deep acquisition of mouse brain transgenically encoded to express YFP in neurons.

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The image shows 8 mm deep acquisition of mouse brain transgenically encoded to express YFP in neurons.

The image shows 8 mm deep acquisition of mouse brain transgenically encoded to express YFP in neurons.

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From Large Scale Imaging to Functional Measurements

From Large Scale Imaging to Functional Measurements

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Motion compensation for Intravital Imaging Microscopy by Dr. Claudio Vinegoni

Motion compensation for Intravital Imaging Microscopy by Dr. Claudio Vinegoni

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Olympus FluoView FV1200 Laser Scanning Confocal Microscope

Olympus FluoView FV1200 Laser Scanning Confocal Microscope

Senior Product Manager Brendan Brinkman discusses the new Olympus FluoView FV1200 and FV1200MPE laser scanning confocal and multiphoton systems and their optimizations for live cell and tissue imaging. Optimized for Imaging Live Cells and Tissues, FV1200 brings sensitivity and power to your research through a range of innovative technology and sensitivity improvements, allowing you to capture the most critical elements of your biological samples with speed, precision and reliability. Topics in the video include new high sensitivity gallium arsenide phosphide (GaAsP) detection, new continuous and one touch autofocus, touch panel control with the IX83 ZDC microscope, improved light throughput with anti-corrosive silver coated galvanometer scanning mirrors, adjustable live cell and animal stage options, near IR and multiphoton laser options, and high speed measurement software and simultaneous stimulation capability for optogenetics.

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