Bioluminescence imaging has been used in a range of research fields over the past decades. Despite its advantages of very low background, high S/N ratios and an extended linear detection range, luminescence imaging with traditional microscopes has suffered from low image resolution and long exposure times. These drawbacks have limited its use and kept bioluminescence in the shadow of fluorescence imaging. With its unique optical design, the LV200 microscope takes full advantage of bioluminescence and provides the capability to acquire images with unrivalled brightness, high resolution and convenience.